Molecular third-harmonic-generation microscopy through resonance enhancement with absorbing dye

Che Hang Yu, Shih Peng Tai, Chun Ta Kung, Wen Jeng Lee, Yi Fan Chan, Hsiang Lin Liu, Jiun Yi Lyu, Chi Kuang Sun

研究成果: 雜誌貢獻文章

31 引文 (Scopus)

摘要

This paper reports a facile and effortless method to realize three-dimensional (3D) molecular third-harmonic-generation (THG) microscopy through the technique of resonance enhancement with absorbing dye. Hematoxylin, a popular absorbing stain, is applied as an example to verify the multiphoton resonant enhancement based on the 1230 nm excitation light and can selectively enhance THG yield at cell nuclear sites in the studied specimens, serving as a cell nucleus contrast agent. It is concluded that combining THG microscopy with the mature staining technique can readily achieve 3D molecular imaging without using fluorescence.

原文英語
頁(從 - 到)387-389
頁數3
期刊Optics Letters
33
發行號4
DOIs
出版狀態已發佈 - 2008 二月 15

指紋

Microscopy
harmonic generations
Coloring Agents
dyes
microscopy
Molecular Imaging
augmentation
Hematoxylin
Cell Nucleus
Contrast Media
Fluorescence
staining
Staining and Labeling
Light
fluorescence
nuclei
cells
excitation

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics

引用此文

Molecular third-harmonic-generation microscopy through resonance enhancement with absorbing dye. / Yu, Che Hang; Tai, Shih Peng; Kung, Chun Ta; Lee, Wen Jeng; Chan, Yi Fan; Liu, Hsiang Lin; Lyu, Jiun Yi; Sun, Chi Kuang.

於: Optics Letters, 卷 33, 編號 4, 15.02.2008, p. 387-389.

研究成果: 雜誌貢獻文章

Yu, CH, Tai, SP, Kung, CT, Lee, WJ, Chan, YF, Liu, HL, Lyu, JY & Sun, CK 2008, 'Molecular third-harmonic-generation microscopy through resonance enhancement with absorbing dye', Optics Letters, 卷 33, 編號 4, 頁 387-389. https://doi.org/10.1364/OL.33.000387
Yu, Che Hang ; Tai, Shih Peng ; Kung, Chun Ta ; Lee, Wen Jeng ; Chan, Yi Fan ; Liu, Hsiang Lin ; Lyu, Jiun Yi ; Sun, Chi Kuang. / Molecular third-harmonic-generation microscopy through resonance enhancement with absorbing dye. 於: Optics Letters. 2008 ; 卷 33, 編號 4. 頁 387-389.
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