Microsatellite loci from Lanyu scops owl (Otus elegans botelensis) and their cross-species application in four species of strigidae

Yu Cheng Hsu; Shou Hsien Li; Yao Sung Lin; Lucia Liu Severinghaus

doi:10.1007/s10592-005-5477-x

Microsatellite loci from Lanyu scops owl (Otus elegans botelensis) and their cross-species application in four species of strigidae

Yu Cheng Hsu, Shou Hsien Li, Yao Sung Lin, Lucia Liu Severinghaus^*

^*此作品的通信作者

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研究成果: 雜誌貢獻 › 期刊論文 › 同行評審

17 引文斯高帕斯（Scopus）

摘要

We developed six new microsatellite markers containing tetranucleotide repeat motifs (GATA/CTAT) for Lanyu scops owl (Otus elegans botelensis) from an enriched partial library. All these loci are polymorphic and conform to Hardy-Weinberg equilibrium. We cross-species tested these and 12 other microsatellite primer pairs previously developed from O. elegans on four other species of owls (O. lettia, O. spilocephalus, O. scops, and Ninox scutulata). Results showed that the degree of polymorphism decreased with increasing phylogenetic distance to O. elegans. Most loci (66.7, 83.3, and 100%) were polymorphic in the three Otus owls but only five (27.8%) were polymorphic in N. scutulata. These microsatellites should be very useful genetic markers in studying the mating system, population genetics, and conservation of other little studied Old World Otus owls.

原文	英語
頁（從 - 到）	161-165
頁數	5
期刊	Conservation Genetics
卷	7
發行號	1
DOIs	https://doi.org/10.1007/s10592-005-5477-x
出版狀態	已發佈 - 2006 2月

ASJC Scopus subject areas

生態學、進化論、行為學與系統學
遺傳學

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10.1007/s10592-005-5477-x

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title = "Microsatellite loci from Lanyu scops owl (Otus elegans botelensis) and their cross-species application in four species of strigidae",

abstract = "We developed six new microsatellite markers containing tetranucleotide repeat motifs (GATA/CTAT) for Lanyu scops owl (Otus elegans botelensis) from an enriched partial library. All these loci are polymorphic and conform to Hardy-Weinberg equilibrium. We cross-species tested these and 12 other microsatellite primer pairs previously developed from O. elegans on four other species of owls (O. lettia, O. spilocephalus, O. scops, and Ninox scutulata). Results showed that the degree of polymorphism decreased with increasing phylogenetic distance to O. elegans. Most loci (66.7, 83.3, and 100%) were polymorphic in the three Otus owls but only five (27.8%) were polymorphic in N. scutulata. These microsatellites should be very useful genetic markers in studying the mating system, population genetics, and conservation of other little studied Old World Otus owls.",

keywords = "DNA polymorphism, Genotyping, Microsatellite, Microsatellites, Otus elegans, Strigidae",

author = "Hsu, {Yu Cheng} and Li, {Shou Hsien} and Lin, {Yao Sung} and Severinghaus, {Lucia Liu}",

note = "Funding Information: We thank members of the Lanyu scops owl research team for collecting O. e. botelensis blood samples, Prof. J.C. Chang, Prof. J. Song and Mr. H.H. Chiu for providing blood samples from other species of owls, Dr. Y.J. Huang and members of Genetic Diversity Laboratory, Department of Life Science, National Taiwan Normal University for help in lab work. This study was funded by a grant from Academia Sinica, Taipei, Taiwan, a National Science Council grant to LLS (NSC 90-2313-B-001-036), and a grant to SHL from the Council of Agriculture [COA 90AT-1.3.3-FR1(5)].",

year = "2006",

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doi = "10.1007/s10592-005-5477-x",

language = "English",

volume = "7",

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T1 - Microsatellite loci from Lanyu scops owl (Otus elegans botelensis) and their cross-species application in four species of strigidae

AU - Hsu, Yu Cheng

AU - Li, Shou Hsien

AU - Lin, Yao Sung

AU - Severinghaus, Lucia Liu

N1 - Funding Information: We thank members of the Lanyu scops owl research team for collecting O. e. botelensis blood samples, Prof. J.C. Chang, Prof. J. Song and Mr. H.H. Chiu for providing blood samples from other species of owls, Dr. Y.J. Huang and members of Genetic Diversity Laboratory, Department of Life Science, National Taiwan Normal University for help in lab work. This study was funded by a grant from Academia Sinica, Taipei, Taiwan, a National Science Council grant to LLS (NSC 90-2313-B-001-036), and a grant to SHL from the Council of Agriculture [COA 90AT-1.3.3-FR1(5)].

PY - 2006/2

Y1 - 2006/2

N2 - We developed six new microsatellite markers containing tetranucleotide repeat motifs (GATA/CTAT) for Lanyu scops owl (Otus elegans botelensis) from an enriched partial library. All these loci are polymorphic and conform to Hardy-Weinberg equilibrium. We cross-species tested these and 12 other microsatellite primer pairs previously developed from O. elegans on four other species of owls (O. lettia, O. spilocephalus, O. scops, and Ninox scutulata). Results showed that the degree of polymorphism decreased with increasing phylogenetic distance to O. elegans. Most loci (66.7, 83.3, and 100%) were polymorphic in the three Otus owls but only five (27.8%) were polymorphic in N. scutulata. These microsatellites should be very useful genetic markers in studying the mating system, population genetics, and conservation of other little studied Old World Otus owls.

AB - We developed six new microsatellite markers containing tetranucleotide repeat motifs (GATA/CTAT) for Lanyu scops owl (Otus elegans botelensis) from an enriched partial library. All these loci are polymorphic and conform to Hardy-Weinberg equilibrium. We cross-species tested these and 12 other microsatellite primer pairs previously developed from O. elegans on four other species of owls (O. lettia, O. spilocephalus, O. scops, and Ninox scutulata). Results showed that the degree of polymorphism decreased with increasing phylogenetic distance to O. elegans. Most loci (66.7, 83.3, and 100%) were polymorphic in the three Otus owls but only five (27.8%) were polymorphic in N. scutulata. These microsatellites should be very useful genetic markers in studying the mating system, population genetics, and conservation of other little studied Old World Otus owls.

KW - DNA polymorphism

KW - Genotyping

KW - Microsatellite

KW - Microsatellites

KW - Otus elegans

KW - Strigidae

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Microsatellite loci from Lanyu scops owl (Otus elegans botelensis) and their cross-species application in four species of strigidae

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