Methanol plug assisted sweeping-micellar electrokinetic chromatography for the determination of dopamine in urine by violet light emitting diode-induced fluorescence detection

Hsuan Ming Huang; Cheng Huang Lin

doi:10.1016/j.jchromb.2004.11.018

Methanol plug assisted sweeping-micellar electrokinetic chromatography for the determination of dopamine in urine by violet light emitting diode-induced fluorescence detection

Hsuan Ming Huang
, Cheng Huang Lin^*

^*此作品的通信作者

化學系

研究成果: 雜誌貢獻 › 期刊論文 › 同行評審

44 引文斯高帕斯（Scopus）

摘要

The use and limitations of a methanol plug assisted sweeping-micellar electrokinetic chromatography (sweeping-MEKC) method is described. Using naphthalene-2,3-dicarboxaldehyde (NDA)-labeled dopamine as a model compound, this new method was also used in the determination of dopamine in actual urine samples. An inexpensive violet light emitting diode (LED) was used for the light source, because this is suitable for fluorescence excitation. The number of theoretical plates of the analyte was determined to be ∼1 × 10 ⁵ and ∼2 × 10⁵ by means of MEKC and sweeping-MEKC and this was improved to ∼1 × 10⁶ when the methanol plug assisted mode was applied. In addition, the detection limit of NDA-labeled dopamine was determined to be 9.1 × 10^-7 and 1.2 × 10^-8 M by means of MEKC and sweeping-MEKC and this was improved to 4.7 × 10^-9 M when the methanol plug assisted sweeping-MEKC mode was applied.

原文	英語
頁（從 - 到）	113-119
頁數	7
期刊	Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
卷	816
發行號	1-2
DOIs	https://doi.org/10.1016/j.jchromb.2004.11.018
出版狀態	已發佈 - 2005 2月 25

ASJC Scopus subject areas

分析化學
生物化學
臨床生物化學
細胞生物學

存取文件

10.1016/j.jchromb.2004.11.018

其它文件與鏈接

引用此

Methanol plug assisted sweeping-micellar electrokinetic chromatography for the determination of dopamine in urine by violet light emitting diode-induced fluorescence detection. / Huang, Hsuan Ming; Lin, Cheng Huang.
於: Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, 卷 816, 編號 1-2, 25.02.2005, p. 113-119.

研究成果: 雜誌貢獻 › 期刊論文 › 同行評審

Huang, HM & Lin, CH 2005, 'Methanol plug assisted sweeping-micellar electrokinetic chromatography for the determination of dopamine in urine by violet light emitting diode-induced fluorescence detection', Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, 卷 816, 編號 1-2, 頁 113-119. https://doi.org/10.1016/j.jchromb.2004.11.018

@article{8122655af507459a8ff35de7aa96b6be,

title = "Methanol plug assisted sweeping-micellar electrokinetic chromatography for the determination of dopamine in urine by violet light emitting diode-induced fluorescence detection",

abstract = "The use and limitations of a methanol plug assisted sweeping-micellar electrokinetic chromatography (sweeping-MEKC) method is described. Using naphthalene-2,3-dicarboxaldehyde (NDA)-labeled dopamine as a model compound, this new method was also used in the determination of dopamine in actual urine samples. An inexpensive violet light emitting diode (LED) was used for the light source, because this is suitable for fluorescence excitation. The number of theoretical plates of the analyte was determined to be ∼1 × 10 5 and ∼2 × 105 by means of MEKC and sweeping-MEKC and this was improved to ∼1 × 106 when the methanol plug assisted mode was applied. In addition, the detection limit of NDA-labeled dopamine was determined to be 9.1 × 10-7 and 1.2 × 10-8 M by means of MEKC and sweeping-MEKC and this was improved to 4.7 × 10-9 M when the methanol plug assisted sweeping-MEKC mode was applied.",

keywords = "Capillary electrophoresis, Dopamine, Methanol plug, Sweeping-MEKC, Violet light emitting diode",

author = "Huang, \{Hsuan Ming\} and Lin, \{Cheng Huang\}",

note = "Funding Information: This work was supported by a grant from the National Science Council of Taiwan under Contract No. NSC-92-2113-M-003-023.",

year = "2005",

month = feb,

day = "25",

doi = "10.1016/j.jchromb.2004.11.018",

language = "English",

volume = "816",

pages = "113--119",

journal = "Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences",

issn = "1570-0232",

publisher = "Elsevier BV",

number = "1-2",

}

TY - JOUR

T1 - Methanol plug assisted sweeping-micellar electrokinetic chromatography for the determination of dopamine in urine by violet light emitting diode-induced fluorescence detection

AU - Huang, Hsuan Ming

AU - Lin, Cheng Huang

N1 - Funding Information: This work was supported by a grant from the National Science Council of Taiwan under Contract No. NSC-92-2113-M-003-023.

PY - 2005/2/25

Y1 - 2005/2/25

N2 - The use and limitations of a methanol plug assisted sweeping-micellar electrokinetic chromatography (sweeping-MEKC) method is described. Using naphthalene-2,3-dicarboxaldehyde (NDA)-labeled dopamine as a model compound, this new method was also used in the determination of dopamine in actual urine samples. An inexpensive violet light emitting diode (LED) was used for the light source, because this is suitable for fluorescence excitation. The number of theoretical plates of the analyte was determined to be ∼1 × 10 5 and ∼2 × 105 by means of MEKC and sweeping-MEKC and this was improved to ∼1 × 106 when the methanol plug assisted mode was applied. In addition, the detection limit of NDA-labeled dopamine was determined to be 9.1 × 10-7 and 1.2 × 10-8 M by means of MEKC and sweeping-MEKC and this was improved to 4.7 × 10-9 M when the methanol plug assisted sweeping-MEKC mode was applied.

AB - The use and limitations of a methanol plug assisted sweeping-micellar electrokinetic chromatography (sweeping-MEKC) method is described. Using naphthalene-2,3-dicarboxaldehyde (NDA)-labeled dopamine as a model compound, this new method was also used in the determination of dopamine in actual urine samples. An inexpensive violet light emitting diode (LED) was used for the light source, because this is suitable for fluorescence excitation. The number of theoretical plates of the analyte was determined to be ∼1 × 10 5 and ∼2 × 105 by means of MEKC and sweeping-MEKC and this was improved to ∼1 × 106 when the methanol plug assisted mode was applied. In addition, the detection limit of NDA-labeled dopamine was determined to be 9.1 × 10-7 and 1.2 × 10-8 M by means of MEKC and sweeping-MEKC and this was improved to 4.7 × 10-9 M when the methanol plug assisted sweeping-MEKC mode was applied.

KW - Capillary electrophoresis

KW - Dopamine

KW - Methanol plug

KW - Sweeping-MEKC

KW - Violet light emitting diode

UR - https://www.scopus.com/pages/publications/12444308401

UR - https://www.scopus.com/pages/publications/12444308401#tab=citedBy

U2 - 10.1016/j.jchromb.2004.11.018

DO - 10.1016/j.jchromb.2004.11.018

M3 - Article

C2 - 15664341

AN - SCOPUS:12444308401

SN - 1570-0232

VL - 816

SP - 113

EP - 119

JO - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences

JF - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences

IS - 1-2

ER -

Methanol plug assisted sweeping-micellar electrokinetic chromatography for the determination of dopamine in urine by violet light emitting diode-induced fluorescence detection

摘要

ASJC Scopus subject areas

存取文件

其它文件與鏈接

指紋

引用此