Measuring the elasticity of ribonucleotide(S)-containing DNA molecules using AFM

Kyung Duk Koh, Hsiang Chih Chiu, Elisa Riedo, Francesca Storici

研究成果: 雜誌貢獻期刊論文同行評審

7 引文 斯高帕斯(Scopus)


Ribonucleotides, ribonucleoside monophosphates (rNMPs), have been revealed as possibly the most noncanonical nucleotides in genomic DNA. rNMPs, either not removed from Okazaki fragments during DNA replication or incorporated and scattered throughout the genome, pose a perturbation to the structure and a threat to the stability of DNA. The instability of DNA is mainly due to the extra 2′-hydroxyl (OH) group of rNMPs which give rise to local structural effects, which may disturb various molecular interactions in cells. As a result of these structural perturbations by rNMPs, the elastic properties of DNA are also affected. Here, we show the approach to test whether the presence of rNMPs in DNA duplexes could alter the elasticity of DNA by implementing atomic force microscopy (AFM)-based single molecule force-measurements of short rNMP(s)-containing oligonucleotides (oligos).

頁(從 - 到)43-57
期刊Methods in Molecular Biology
出版狀態已發佈 - 2015

ASJC Scopus subject areas

  • 分子生物學
  • 遺傳學


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