Magnetic nanoparticles for high-sensitivity detection on nucleic acids via superconducting-quantum-interference-device-based immunomagnetic reduction assay

S. Y. Yang; J. J. Chieh; W. C. Wang; C. Y. Yu; N. S. Hing; H. E. Horng; Chin Yih Hong; H. C. Yang; C. F. Chang; H. Y. Lin

doi:10.1016/j.jmmm.2010.10.011

Magnetic nanoparticles for high-sensitivity detection on nucleic acids via superconducting-quantum-interference-device-based immunomagnetic reduction assay

S. Y. Yang, J. J. Chieh, W. C. Wang, C. Y. Yu, N. S. Hing, H. E. Horng, Chin Yih Hong, H. C. Yang, C. F. Chang, H. Y. Lin

研究成果: 雜誌貢獻 › 期刊論文 › 同行評審

39 引文斯高帕斯（Scopus）

摘要

In this work, we investigate the feasibility of detecting quantitatively DNA molecules utilizing the technology named after the immunomagnetic reduction (IMR) assay. Magnetic nanoparticles dispersed in a phosphate buffer saline solution were bio-functionalized with probing single-strand DNA. A superconducting quantum interference device (SQUID) ac magnetosusceptometer was employed to detect IMR signals related to the concentration of the target DNA. The results reveal that use of IMR assay had merits such as a high convenience level, e.g. wash-free processes and high sensitivity, down to pM, for DNA detection.

原文	英語
頁（從 - 到）	681-685
頁數	5
期刊	Journal of Magnetism and Magnetic Materials
卷	323
發行號	6
DOIs	https://doi.org/10.1016/j.jmmm.2010.10.011
出版狀態	已發佈 - 2011 3月

ASJC Scopus subject areas

電子、光磁材料
凝聚態物理學

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10.1016/j.jmmm.2010.10.011

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Yang, S. Y., Chieh, J. J., Wang, W. C., Yu, C. Y., Hing, N. S., Horng, H. E., Hong, C. Y., Yang, H. C., Chang, C. F., & Lin, H. Y. (2011). Magnetic nanoparticles for high-sensitivity detection on nucleic acids via superconducting-quantum-interference-device-based immunomagnetic reduction assay. Journal of Magnetism and Magnetic Materials, 323(6), 681-685. https://doi.org/10.1016/j.jmmm.2010.10.011

Yang, SY, Chieh, JJ, Wang, WC, Yu, CY, Hing, NS, Horng, HE, Hong, CY, Yang, HC, Chang, CF & Lin, HY 2011, 'Magnetic nanoparticles for high-sensitivity detection on nucleic acids via superconducting-quantum-interference-device-based immunomagnetic reduction assay', Journal of Magnetism and Magnetic Materials, 卷 323, 編號 6, 頁 681-685. https://doi.org/10.1016/j.jmmm.2010.10.011

@article{e63b5d7b51814287b27e0d1d8c6031cd,

title = "Magnetic nanoparticles for high-sensitivity detection on nucleic acids via superconducting-quantum-interference-device-based immunomagnetic reduction assay",

abstract = "In this work, we investigate the feasibility of detecting quantitatively DNA molecules utilizing the technology named after the immunomagnetic reduction (IMR) assay. Magnetic nanoparticles dispersed in a phosphate buffer saline solution were bio-functionalized with probing single-strand DNA. A superconducting quantum interference device (SQUID) ac magnetosusceptometer was employed to detect IMR signals related to the concentration of the target DNA. The results reveal that use of IMR assay had merits such as a high convenience level, e.g. wash-free processes and high sensitivity, down to pM, for DNA detection.",

keywords = "DNA, Immunomagnetic reduction, Magnetic nanoparticle",

author = "Yang, {S. Y.} and Chieh, {J. J.} and Wang, {W. C.} and Yu, {C. Y.} and Hing, {N. S.} and Horng, {H. E.} and Hong, {Chin Yih} and Yang, {H. C.} and Chang, {C. F.} and Lin, {H. Y.}",

note = "Funding Information: This work is supported by the National Science Council of Taiwan under Grant nos. NSC98-2323-B-003-001-CC2 , 98-2112-M003-003 , and 98-2752-M-002-016-PAE .",

year = "2011",

month = mar,

doi = "10.1016/j.jmmm.2010.10.011",

language = "English",

volume = "323",

pages = "681--685",

journal = "Journal of Magnetism and Magnetic Materials",

issn = "0304-8853",

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TY - JOUR

T1 - Magnetic nanoparticles for high-sensitivity detection on nucleic acids via superconducting-quantum-interference-device-based immunomagnetic reduction assay

AU - Yang, S. Y.

AU - Chieh, J. J.

AU - Wang, W. C.

AU - Yu, C. Y.

AU - Hing, N. S.

AU - Horng, H. E.

AU - Hong, Chin Yih

AU - Yang, H. C.

AU - Chang, C. F.

AU - Lin, H. Y.

N1 - Funding Information: This work is supported by the National Science Council of Taiwan under Grant nos. NSC98-2323-B-003-001-CC2 , 98-2112-M003-003 , and 98-2752-M-002-016-PAE .

PY - 2011/3

Y1 - 2011/3

N2 - In this work, we investigate the feasibility of detecting quantitatively DNA molecules utilizing the technology named after the immunomagnetic reduction (IMR) assay. Magnetic nanoparticles dispersed in a phosphate buffer saline solution were bio-functionalized with probing single-strand DNA. A superconducting quantum interference device (SQUID) ac magnetosusceptometer was employed to detect IMR signals related to the concentration of the target DNA. The results reveal that use of IMR assay had merits such as a high convenience level, e.g. wash-free processes and high sensitivity, down to pM, for DNA detection.

AB - In this work, we investigate the feasibility of detecting quantitatively DNA molecules utilizing the technology named after the immunomagnetic reduction (IMR) assay. Magnetic nanoparticles dispersed in a phosphate buffer saline solution were bio-functionalized with probing single-strand DNA. A superconducting quantum interference device (SQUID) ac magnetosusceptometer was employed to detect IMR signals related to the concentration of the target DNA. The results reveal that use of IMR assay had merits such as a high convenience level, e.g. wash-free processes and high sensitivity, down to pM, for DNA detection.

KW - DNA

KW - Immunomagnetic reduction

KW - Magnetic nanoparticle

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U2 - 10.1016/j.jmmm.2010.10.011

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EP - 685

JO - Journal of Magnetism and Magnetic Materials

JF - Journal of Magnetism and Magnetic Materials

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ER -

Magnetic nanoparticles for high-sensitivity detection on nucleic acids via superconducting-quantum-interference-device-based immunomagnetic reduction assay

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