Magnetic clustering effect during the association of biofunctionalized magnetic nanoparticles with biomarkers

Kuen Lin Chen, Jean Hong Chen, Su Hsien Liao, Jen Je Chieh, Herng-Er Horng, Li Min Wang, Hong-Chang Yang

研究成果: 雜誌貢獻期刊論文同行評審

11 引文 斯高帕斯(Scopus)

摘要

We report herein an investigation into dynamic magnetic clustering that occurs during immunoassays as biofunctionalized magnetic nanoparticles (BMNs) become associated with biotargets. We measure the dynamic effective relaxation time Teff(f) and use scanning electron microscopy (SEM) and transmission electron microscopy (TEM) to investigate the C-reactive protein (CRP) as it associates with the BMN Fe3O4-antiCRP to form the magnetic cluster Fe3O4-antiCRP-CRP. The results indicate that Teff(f) increases with increasing association time. In addition, the ration ΔTeff/T0as a function of CRP concentration follows a characteristic logistic function, which provides a basis for estimating the quantity of biomole-cules with a detection sensitivity close to 0.1 ppm. After the association, SEM and TEM images show that CRP and Fe3O4-antiCRP conjugate to form Fe3O4-antiCRP-CRP clusters hundreds of nanometers in size. The SEM and TEM images provide direct evidence of the formation of magnetic clustering.

原文英語
文章編號e0135290
期刊PloS one
10
發行號8
DOIs
出版狀態已發佈 - 2015 八月 13

ASJC Scopus subject areas

  • 生物化學、遺傳與分子生物學 (全部)
  • 農業與生物科學 (全部)
  • 多學科

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