Magnetic clustering effect during the association of biofunctionalized magnetic nanoparticles with biomarkers

Kuen Lin Chen, Jean Hong Chen, Su Hsien Liao, Jen Je Chieh, Herng Er Horng, Li Min Wang, Hong Chang Yang

研究成果: 雜誌貢獻文章

9 引文 斯高帕斯(Scopus)


We report herein an investigation into dynamic magnetic clustering that occurs during immunoassays as biofunctionalized magnetic nanoparticles (BMNs) become associated with biotargets. We measure the dynamic effective relaxation time Teff(f) and use scanning electron microscopy (SEM) and transmission electron microscopy (TEM) to investigate the C-reactive protein (CRP) as it associates with the BMN Fe3O4-antiCRP to form the magnetic cluster Fe3O4-antiCRP-CRP. The results indicate that Teff(f) increases with increasing association time. In addition, the ration ΔTeff/T0as a function of CRP concentration follows a characteristic logistic function, which provides a basis for estimating the quantity of biomole-cules with a detection sensitivity close to 0.1 ppm. After the association, SEM and TEM images show that CRP and Fe3O4-antiCRP conjugate to form Fe3O4-antiCRP-CRP clusters hundreds of nanometers in size. The SEM and TEM images provide direct evidence of the formation of magnetic clustering.

期刊PloS one
出版狀態已發佈 - 2015 八月 13


ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • General