Isolation and characterization of microsatellite markers in Tsaiya duck

M. C. Hsiao, H. C. Liu*, Y. C. Hsu, Y. H. Hu, S. H. Li, S. R. Lee


研究成果: 雜誌貢獻期刊論文同行評審

8 引文 斯高帕斯(Scopus)


An enrichment library of GATA-repeats from genomic DNA was constructed in this study to isolate and characterize microsatellite loci in Tsaiya duck (Anas platyrhynchos). Thirty-three microsatellite markers were developed and used to detect polymorphisms in 30 Tsaiya ducks. A total of 177 alleles were observed and all loci except APT022 were polymorphic. The number of alleles ranged from 2 to 9 with an average of 5.5 per microsatellite locus. The observed and expected heterozygosity of these polymorphic markers ranged from 0.07 to 0.93 with an average number of 0.60 and 0.10 to 0.86 with an average number of 0.61, respectively. Among the polymorphic markers, the observed heterozygosities of 23 loci were higher than 0.50 (69.70%). The polymorphism information content (PIC) in the 32 loci ranged from 0.09 to 0.83 with an average of 0.57. Seven of the 33 duck microsatellite loci had orthologs in the chicken genome, but only APT004 had a similar core repeat to chickens. These microsatellite markers will be useful in constructing a genetic linkage map for the duck and a comparative mapping with the chicken can also provide a valuable tool for studies related to biodiversity and population genetics in this duck species.

頁(從 - 到)624-627
期刊Asian-Australasian Journal of Animal Sciences
出版狀態已發佈 - 2008 5月

ASJC Scopus subject areas

  • 食品科學
  • 動物科學與動物學
  • 工程 (全部)


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