Isolation and characterization of microsatellite DNA markers from the Lanyu scops owl (Otus elegans botelensis)

Yu Cheng Hsu; Lucia L. Severinghaus; Yao Sung Lin; Shou Hsien Li

doi:10.1046/j.1471-8286.2003.00523.x

Isolation and characterization of microsatellite DNA markers from the Lanyu scops owl (Otus elegans botelensis)

Yu Cheng Hsu, Lucia L. Severinghaus, Yao Sung Lin, Shou Hsien Li^*

^*此作品的通信作者

生命科學系

研究成果: 雜誌貢獻 › 期刊論文 › 同行評審

26 引文斯高帕斯（Scopus）

摘要

From a genomic library enriched for GATA/CTAT and GAAA/CTTT repeats, 12 polymorphic microsatellite markers were developed for the Lanyu scops owl (Otus elegans botelensis). Polymorphism of these loci was evaluated in a sample of 58 adult individuals of unknown relationship. The allele numbers of each locus were from five to 25 and the observed heterozygosity of each locus ranges from 0.707 to 0.914. When using these loci in parentage assignment, the probability of false parent exclusion is greater than 0.999. All loci conformed to Hardy-Weinberg expectations.

原文	英語
頁（從 - 到）	595-597
頁數	3
期刊	Molecular Ecology Notes
卷	3
發行號	4
DOIs	https://doi.org/10.1046/j.1471-8286.2003.00523.x
出版狀態	已發佈 - 2003 12月

ASJC Scopus subject areas

生物化學
生態學
一般生物化學，遺傳學和分子生物學

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10.1046/j.1471-8286.2003.00523.x

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title = "Isolation and characterization of microsatellite DNA markers from the Lanyu scops owl (Otus elegans botelensis)",

abstract = "From a genomic library enriched for GATA/CTAT and GAAA/CTTT repeats, 12 polymorphic microsatellite markers were developed for the Lanyu scops owl (Otus elegans botelensis). Polymorphism of these loci was evaluated in a sample of 58 adult individuals of unknown relationship. The allele numbers of each locus were from five to 25 and the observed heterozygosity of each locus ranges from 0.707 to 0.914. When using these loci in parentage assignment, the probability of false parent exclusion is greater than 0.999. All loci conformed to Hardy-Weinberg expectations.",

keywords = "Enrichment, Genotyping, Lanyu scops owl, Microsatellites, Otus elegans botelensis, Strigidae",

author = "Hsu, {Yu Cheng} and Severinghaus, {Lucia L.} and Lin, {Yao Sung} and Li, {Shou Hsien}",

year = "2003",

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doi = "10.1046/j.1471-8286.2003.00523.x",

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AU - Hsu, Yu Cheng

AU - Severinghaus, Lucia L.

AU - Lin, Yao Sung

AU - Li, Shou Hsien

PY - 2003/12

Y1 - 2003/12

N2 - From a genomic library enriched for GATA/CTAT and GAAA/CTTT repeats, 12 polymorphic microsatellite markers were developed for the Lanyu scops owl (Otus elegans botelensis). Polymorphism of these loci was evaluated in a sample of 58 adult individuals of unknown relationship. The allele numbers of each locus were from five to 25 and the observed heterozygosity of each locus ranges from 0.707 to 0.914. When using these loci in parentage assignment, the probability of false parent exclusion is greater than 0.999. All loci conformed to Hardy-Weinberg expectations.

AB - From a genomic library enriched for GATA/CTAT and GAAA/CTTT repeats, 12 polymorphic microsatellite markers were developed for the Lanyu scops owl (Otus elegans botelensis). Polymorphism of these loci was evaluated in a sample of 58 adult individuals of unknown relationship. The allele numbers of each locus were from five to 25 and the observed heterozygosity of each locus ranges from 0.707 to 0.914. When using these loci in parentage assignment, the probability of false parent exclusion is greater than 0.999. All loci conformed to Hardy-Weinberg expectations.

KW - Enrichment

KW - Genotyping

KW - Lanyu scops owl

KW - Microsatellites

KW - Otus elegans botelensis

KW - Strigidae

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U2 - 10.1046/j.1471-8286.2003.00523.x

DO - 10.1046/j.1471-8286.2003.00523.x

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VL - 3

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JO - Molecular Ecology Notes

JF - Molecular Ecology Notes

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ER -

Isolation and characterization of microsatellite DNA markers from the Lanyu scops owl (Otus elegans botelensis)

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