Isolation and characterization of 18 microsatellite loci for an endangered fresh water crocodile Tomistoma schlegelii

Yi Chun Chang; Shou Hsien Li; Ming Hsung Chang; Szu Lung Chen

doi:10.1007/s12686-013-0139-7

Isolation and characterization of 18 microsatellite loci for an endangered fresh water crocodile Tomistoma schlegelii

Yi Chun Chang, Shou Hsien Li, Ming Hsung Chang, Szu Lung Chen^*

^*此作品的通信作者

生命科學系

研究成果: 雜誌貢獻 › 通訊期刊論文 › 同行評審

3 引文斯高帕斯（Scopus）

摘要

Tomistoma schlegelii is an endangered crocodilian with an adult population size less than 2,500 individuals. To make appropriate in situ and ex situ conservation strategies, it is essential to perform parentage assignment for breeding captive populations and to conduct population study in wild populations based on genetic data. However, there is no suitable genetic marker available for T. schlegelii. Here, we reported 18 novel microsatellite loci from the tomistoma. Allele numbers of these loci ranged from 2 to 16, and their observed heterozygosity ranged from 0.105 to 0.684. The probability for excluding false parental pairs using the 18 loci was 99.9999 %. These markers are useful not only in parentage assignment but also in population structure analysis.

原文	英語
頁（從 - 到）	495-498
頁數	4
期刊	Conservation Genetics Resources
卷	6
發行號	2
DOIs	https://doi.org/10.1007/s12686-013-0139-7
出版狀態	已發佈 - 2014 6月

ASJC Scopus subject areas

生態學、進化論、行為學與系統學
遺傳學

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10.1007/s12686-013-0139-7

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title = "Isolation and characterization of 18 microsatellite loci for an endangered fresh water crocodile Tomistoma schlegelii",

abstract = "Tomistoma schlegelii is an endangered crocodilian with an adult population size less than 2,500 individuals. To make appropriate in situ and ex situ conservation strategies, it is essential to perform parentage assignment for breeding captive populations and to conduct population study in wild populations based on genetic data. However, there is no suitable genetic marker available for T. schlegelii. Here, we reported 18 novel microsatellite loci from the tomistoma. Allele numbers of these loci ranged from 2 to 16, and their observed heterozygosity ranged from 0.105 to 0.684. The probability for excluding false parental pairs using the 18 loci was 99.9999 %. These markers are useful not only in parentage assignment but also in population structure analysis.",

keywords = "Crocodilian, False gharial, Gavialidae, Microsatellite",

author = "Chang, {Yi Chun} and Li, {Shou Hsien} and Chang, {Ming Hsung} and Chen, {Szu Lung}",

note = "Funding Information: Acknowledgments We thank Lung-Qun Crocodile farm and Wanpi World Safari Zoo for providing T. schlegelii samples. We thank Dr. Chih-Ming Hung for manuscript proofreading. We also thank many colleagues in Taipei Zoo for help in sample collection. This study was funded by the Animal Adoption Program of Taipei Zoo and the",

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doi = "10.1007/s12686-013-0139-7",

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AU - Chang, Yi Chun

AU - Li, Shou Hsien

AU - Chang, Ming Hsung

AU - Chen, Szu Lung

N1 - Funding Information: Acknowledgments We thank Lung-Qun Crocodile farm and Wanpi World Safari Zoo for providing T. schlegelii samples. We thank Dr. Chih-Ming Hung for manuscript proofreading. We also thank many colleagues in Taipei Zoo for help in sample collection. This study was funded by the Animal Adoption Program of Taipei Zoo and the

PY - 2014/6

Y1 - 2014/6

N2 - Tomistoma schlegelii is an endangered crocodilian with an adult population size less than 2,500 individuals. To make appropriate in situ and ex situ conservation strategies, it is essential to perform parentage assignment for breeding captive populations and to conduct population study in wild populations based on genetic data. However, there is no suitable genetic marker available for T. schlegelii. Here, we reported 18 novel microsatellite loci from the tomistoma. Allele numbers of these loci ranged from 2 to 16, and their observed heterozygosity ranged from 0.105 to 0.684. The probability for excluding false parental pairs using the 18 loci was 99.9999 %. These markers are useful not only in parentage assignment but also in population structure analysis.

AB - Tomistoma schlegelii is an endangered crocodilian with an adult population size less than 2,500 individuals. To make appropriate in situ and ex situ conservation strategies, it is essential to perform parentage assignment for breeding captive populations and to conduct population study in wild populations based on genetic data. However, there is no suitable genetic marker available for T. schlegelii. Here, we reported 18 novel microsatellite loci from the tomistoma. Allele numbers of these loci ranged from 2 to 16, and their observed heterozygosity ranged from 0.105 to 0.684. The probability for excluding false parental pairs using the 18 loci was 99.9999 %. These markers are useful not only in parentage assignment but also in population structure analysis.

KW - Crocodilian

KW - False gharial

KW - Gavialidae

KW - Microsatellite

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Isolation and characterization of 18 microsatellite loci for an endangered fresh water crocodile Tomistoma schlegelii

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