Independent modulation of Arabidopsis thaliana polyubiquitin mRNAs in different organs and in response to environmental changes

Chih Wen Sun, Judy Callis*

*此作品的通信作者

研究成果: 雜誌貢獻期刊論文同行評審

101 引文 斯高帕斯(Scopus)

摘要

The highly conserved protein ubiquitin is encoded by five polyubiquitin genes in Arabidopsis thaliana ecotype Columbia that have been divided into two subtypes, the UBQ3/UBQ4 subtype and the UBQ10/UBQ11/UBQ14 subtype. Northern analysis using gene-specific oligonucleotides as hybridization probes and enzyme activity measurements from transgenic plants expressing β-glucuronidase (GUS) under the control of individual polyubiquitin 5' flanking regions were used to determine the developmental and environmental regulation of polyubiquitin transcription and mRNA accumulation. Polyubiquitin mRNA levels within and between subtypes were independently modulated. UBQ3 mRNA levels were threefold higher than UBQ4 mRNA levels in vegetative organs, but only two-thirds of the UBQ4 mRNA levels in flowers, UBQ3 mRNA was modulated by dark/light treatments, while mRNAs from UBQ4 and all members of the other subtype were unaffected. Similarly, within the UBQ10/UBQ11/UBQ14 subtype, UBQ11/UBQ14 mRNAs were modulated differently in seedlings after a two-hour heat-shock treatment. Among all the polyubiquitin genes, UBQ10 mRNA level was the most constant in all organs and environmental conditions examined. Transgenic plants transformed with a UBQ10 5' flanking region::GUS gene contained higher levels of GUS activity than transgenic plants expressing GUS under the control of UBQ3 5' flanking regions. In conclusion, the relative abundance of different Arabidopsis polyubiquitin mRNAs, even those produced from highly similar genes within a subtype, appears to be modulated independently in response to developmental and environmental cues.

原文英語
頁(從 - 到)1017-1027
頁數11
期刊Plant Journal
11
發行號5
DOIs
出版狀態已發佈 - 1997 5月
對外發佈

ASJC Scopus subject areas

  • 遺傳學
  • 植物科學
  • 細胞生物學

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