In vitro characterization and in vivo application of a dual functional peptide

Chien Jung Chen, Ping Hsueh Kuo, Ta Jen Hung, Shun Lung Fang, Che Chuan Yang, Shieh Yueh Yang, Margaret Dah Tsyr Chang*

*此作品的通信作者

研究成果: 書貢獻/報告類型會議論文篇章

1 引文 斯高帕斯(Scopus)

摘要

Heparan sulfate (HS), one kind of sulfated glycosaminoglycans (GAGs), plays an important role in growth factor-receptor interaction, thereby involving in growth, angiogenesis and innate immune signaling. It is also highly addressed that GAGs and their anchoring core proteins are abnormally expressed in various cancers including lung, liver, and colon cancers. Human ribonuclease A (RNaseA) family members, hRNase2, hRNase3 and hRNase5 are reported to bind to cell surface by recognition of sulfated GAGs. We have recently identified three functionally important HS and heparin binding regions in hRNase3, also named as human eosinophil cationic protein (ECP), and characterized a dual-functional peptide (CPPecp) with GAG-binding and cell-penetrating activities. Screening of in vitro binding activity on various gastrointestinal cell lines demonstrated that CPPecp bound to cells rich in HS on the cell surface. However, cellular binding activity of FITC-CPPecp was abolished while cell surface HS was removed by haparinase treatment. In order to characterize the specific binding of CPPecp, quantitative assay was carried out to precisely decipher binding affinity between CPPecp and a variety of GAGs. In addition, magnetic nanoparticle (Fe3O4)-conjugated CPPecp (MNP-CPPecp) was synthesized as a novel bioprobe to evaluate its feasibility in in vivo magnetic resonance imaging (MRI) analysis in colon tumor mouse model. Taken together, our CPPecp in vitro preferred to bind to sulfated GAGs and selectively attach to HS on cell surface. Moreover, CPPecp demonstrated in vivo tumor targeting activity in colon tumor mouse model, suggesting that CPPecp possesses high potential to be developed as a novel molecular imaging agent and cancer targeting delivery vector.

原文英語
主出版物標題Proceedings - 2013 7th International Conference on Complex, Intelligent, and Software Intensive Systems, CISIS 2013
頁面576-581
頁數6
DOIs
出版狀態已發佈 - 2013
對外發佈
事件2013 7th International Conference on Complex, Intelligent, and Software Intensive Systems, CISIS 2013 - Taichung, 臺灣
持續時間: 2013 七月 32013 七月 5

出版系列

名字Proceedings - 2013 7th International Conference on Complex, Intelligent, and Software Intensive Systems, CISIS 2013

會議

會議2013 7th International Conference on Complex, Intelligent, and Software Intensive Systems, CISIS 2013
國家/地區臺灣
城市Taichung
期間2013/07/032013/07/05

ASJC Scopus subject areas

  • 人工智慧
  • 軟體

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