TY - JOUR
T1 - Identification of 2,5-dimethoxy-4-ethylthiophenethylamine and its metabolites in the urine of rats by gas chromatography-mass spectrometry
AU - Lin, Li Chan
AU - Liu, Ju Tsung
AU - Chou, Shiu Huey
AU - Lin, Cheng Huang
N1 - Funding Information:
This work was supported by a grant from the National Science Council of Taiwan under Contract No. NSC-91-2113-M-003-016. The authors thank Prof. Ching-Fa Yao for the helpful discussions.
PY - 2003/12/25
Y1 - 2003/12/25
N2 - A simple and specific method based on gas chromatography-selected ion monitoring-mass spectrometry (GC-SIM-MS) for the analysis of in vivo metabolism of 2,5-dimethoxy-4-ethylthiophenethylamine (2C-T-2) in rats is described. Three male rats were administered 20mg/kg of 2C-T-2 by intra-peritoneal injection, and 24h urine fractions were collected before and after the administration for analysis. After acidic hydrolysis of the urine samples, the metabolites were liquid-liquid extraction and analyzed by a quadruple mass spectrometer in the selected ion monitoring mode. The findings show that four metabolites of 2-(4-ethylthio-2,5-dimethoxyphenyl)-ethanol (Mw: 242), 4-ethylthio-2,5-dimethoxyphenyl acetic acid (Mw: 256), 1-acetoamino-2-(2-hydroxy-4-ethylthio-5-methoxyphenyl)-ethane (Mw: 269) and 1-acetoamino-2-(2-methoxy-4-ethylthio-5-hydroxyphenyl)-ethane (M w: 269) are present and the metabolic pathway for 2C-T-2 in the rat is proposed.
AB - A simple and specific method based on gas chromatography-selected ion monitoring-mass spectrometry (GC-SIM-MS) for the analysis of in vivo metabolism of 2,5-dimethoxy-4-ethylthiophenethylamine (2C-T-2) in rats is described. Three male rats were administered 20mg/kg of 2C-T-2 by intra-peritoneal injection, and 24h urine fractions were collected before and after the administration for analysis. After acidic hydrolysis of the urine samples, the metabolites were liquid-liquid extraction and analyzed by a quadruple mass spectrometer in the selected ion monitoring mode. The findings show that four metabolites of 2-(4-ethylthio-2,5-dimethoxyphenyl)-ethanol (Mw: 242), 4-ethylthio-2,5-dimethoxyphenyl acetic acid (Mw: 256), 1-acetoamino-2-(2-hydroxy-4-ethylthio-5-methoxyphenyl)-ethane (Mw: 269) and 1-acetoamino-2-(2-methoxy-4-ethylthio-5-hydroxyphenyl)-ethane (M w: 269) are present and the metabolic pathway for 2C-T-2 in the rat is proposed.
KW - 2,5-Dimethoxy-4-ethylthiophenethylamine
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U2 - 10.1016/j.jchromb.2003.09.052
DO - 10.1016/j.jchromb.2003.09.052
M3 - Article
C2 - 14643503
AN - SCOPUS:0345276753
SN - 1570-0232
VL - 798
SP - 241
EP - 247
JO - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
JF - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
IS - 2
ER -