Hydrolysis catalyzed with a resin containing histidine groups

Wei Hsiu Hung, Cho Chun Hu, Chuen Ying Liu*

*此作品的通信作者

研究成果: 雜誌貢獻期刊論文同行評審

3 引文 斯高帕斯(Scopus)

摘要

A histidine-containing polymer was synthesized in which the amino group of the histidine was attached chemically via an azide coupling method to the carboxylic acid of Amberlite IRC-50. The resultant polymer was applied as a catalyst for hydrolysis of p-nitrophenyl acetate (PNPA). PNPA in aqueous solution was hydrolyzed at 25°C with a phosphate buffer (pH 7.8). The observed kinetics obeyed Michaelis-Menten kinetics. The reaction rates at various temperature were measured. The activation parameters, preexponential factor (A) and activation energy (Ea), were 6.64 × 10-4 min-1 and 37.5 kJ mol-1 respectively. At a pH of the medium greater than 7.8, the reaction rate remained almost constant (kobs = 0.024 min-1) and seemed to be controlled by the rate of diffusion of PNPA from the bulk solution into the catalytically active site at the resin channel surface. For catalysed hydrolysis, the effect of ionic strength in solution demonstrated that bifunctional cooperation between adjacent histidine groups existed through the nucleophilicity of nitrogen. The effects of metal ions and aspartic acid or serine on hydrolysis were also investigated.

原文英語
頁(從 - 到)67-73
頁數7
期刊Journal of Molecular Catalysis A: Chemical
106
發行號1-2
DOIs
出版狀態已發佈 - 1996 一月 1

ASJC Scopus subject areas

  • 催化
  • 製程化學與技術
  • 物理與理論化學

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