Hoxa 11 structure, extensive antisense transcription, and function in male and female fertility

H. M. Hsieh-Li, D. P. Witte, M. Weinstein, W. Branford, H. Li, K. Small, S. S. Potter

研究成果: 雜誌貢獻文章同行評審

345 引文 斯高帕斯(Scopus)

摘要

Hoxa 11 is a murine Abdominal-B-type homeobox gene. The structure of this gene is presented, including genomic and cDNA sequence. The cDNA includes the complete open reading frame and based on primer extension results is near full length. Surprisingly, the antisense strand of Hoxa 11 was found to be transcribed. Moreover, these antisense transcripts were processed and polyadenylated. The developmental expression patterns for both sense and antisense transcripts were examined using serial section and whole-mount in situ hybridizations. Hoxa 11 transcription patterns were defined in the limbs, kidney and stromal cells surrounding the Mullerian and Wolffian ducts. Of particular interest, in the developing limbs, the sense and antisense transcripts showed complementary expression patterns, with antisense RNAs increasing in abundance in regions where sense RNAs were diminishing in abundance. Furthermore, targeted mutation of Hoxa 11 is shown to result in both male and female sterility. The female mutants produce normal ova, which develop properly post-fertilization when transferred to wild-type surrogate mothers. The Hoxa 11 homozygous mutants are shown to provide a defective uterine environment. The mutant males exhibited a malformation of the vas deferens that resembles a partial homeotic transformation to an epididymis. In addition, the mutant testes fail to descend properly into the scrotum and, likely as a result, spermatogenesis is perturbed.

原文英語
頁(從 - 到)1373-1385
頁數13
期刊Development
121
發行號5
出版狀態已發佈 - 1995

ASJC Scopus subject areas

  • Molecular Biology
  • Developmental Biology

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