Goniothalamin inhibits growth of human lung cancer cells through DNA damage, apoptosis, and reduced migration ability

Chien Chih Chiu; Po Len Liu; Kuang Jing Huang; Hui Min Wang; Kuo Feng Chang; Chon Kit Chou; Fang Rong Chang; Inn Wen Chong; Kang Fang; Jia Shing Chen; Hsueh Wei Chang; Yang Chang Wu

doi:10.1021/jf200566a

Goniothalamin inhibits growth of human lung cancer cells through DNA damage, apoptosis, and reduced migration ability

Chien Chih Chiu, Po Len Liu, Kuang Jing Huang, Hui Min Wang, Kuo Feng Chang, Chon Kit Chou, Fang Rong Chang, Inn Wen Chong, Kang Fang, Jia Shing Chen, Hsueh Wei Chang^*, Yang Chang Wu

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生命科學系

研究成果: 雜誌貢獻 › 期刊論文 › 同行評審

74 引文斯高帕斯（Scopus）

摘要

We evaluated the possible anticancer performance of a natural compound, goniothalamin (GTN), against human lung cancer using as a non-small cell lung cancer (NSCLC) cell line, H1299, as the model system. Cellular proliferation was significantly inhibited by GTN. Using an improved alkaline comet-nuclear extract (comet-NE) assay, GTN was found to induce a significant increase in the tail DNA. Wound healing and zymography assays showed that GTN attenuated cell migration and caused a reduction in the activity level of two major migration-associated matrix metalloproteinases, MMP-2 and MMP-9. It can be concluded that the DNA-damaging effect of GTN against lung cancer cells leads to growth inhibition as well as a depression in migration ability. Therefore, GTN has potential as a chemotherapeutic agent against lung cancer.

原文	英語
頁（從 - 到）	4288-4293
頁數	6
期刊	Journal of Agricultural and Food Chemistry
卷	59
發行號	8
DOIs	https://doi.org/10.1021/jf200566a
出版狀態	已發佈 - 2011 4月 27

ASJC Scopus subject areas

一般化學
一般農業與生物科學

UN SDG

此研究成果有助於以下永續發展目標

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10.1021/jf200566a

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Chiu, C. C., Liu, P. L., Huang, K. J., Wang, H. M., Chang, K. F., Chou, C. K., Chang, F. R., Chong, I. W., Fang, K., Chen, J. S., Chang, H. W., & Wu, Y. C. (2011). Goniothalamin inhibits growth of human lung cancer cells through DNA damage, apoptosis, and reduced migration ability. Journal of Agricultural and Food Chemistry, 59(8), 4288-4293. https://doi.org/10.1021/jf200566a

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title = "Goniothalamin inhibits growth of human lung cancer cells through DNA damage, apoptosis, and reduced migration ability",

abstract = "We evaluated the possible anticancer performance of a natural compound, goniothalamin (GTN), against human lung cancer using as a non-small cell lung cancer (NSCLC) cell line, H1299, as the model system. Cellular proliferation was significantly inhibited by GTN. Using an improved alkaline comet-nuclear extract (comet-NE) assay, GTN was found to induce a significant increase in the tail DNA. Wound healing and zymography assays showed that GTN attenuated cell migration and caused a reduction in the activity level of two major migration-associated matrix metalloproteinases, MMP-2 and MMP-9. It can be concluded that the DNA-damaging effect of GTN against lung cancer cells leads to growth inhibition as well as a depression in migration ability. Therefore, GTN has potential as a chemotherapeutic agent against lung cancer.",

keywords = "Goniothalamin, cell migration, comet assay, matrix metalloproteinase, non-small cell lung cancer",

author = "Chiu, {Chien Chih} and Liu, {Po Len} and Huang, {Kuang Jing} and Wang, {Hui Min} and Chang, {Kuo Feng} and Chou, {Chon Kit} and Chang, {Fang Rong} and Chong, {Inn Wen} and Kang Fang and Chen, {Jia Shing} and Chang, {Hsueh Wei} and Wu, {Yang Chang}",

year = "2011",

month = apr,

day = "27",

doi = "10.1021/jf200566a",

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T1 - Goniothalamin inhibits growth of human lung cancer cells through DNA damage, apoptosis, and reduced migration ability

AU - Chiu, Chien Chih

AU - Liu, Po Len

AU - Huang, Kuang Jing

AU - Wang, Hui Min

AU - Chang, Kuo Feng

AU - Chou, Chon Kit

AU - Chang, Fang Rong

AU - Chong, Inn Wen

AU - Fang, Kang

AU - Chen, Jia Shing

AU - Chang, Hsueh Wei

AU - Wu, Yang Chang

PY - 2011/4/27

Y1 - 2011/4/27

N2 - We evaluated the possible anticancer performance of a natural compound, goniothalamin (GTN), against human lung cancer using as a non-small cell lung cancer (NSCLC) cell line, H1299, as the model system. Cellular proliferation was significantly inhibited by GTN. Using an improved alkaline comet-nuclear extract (comet-NE) assay, GTN was found to induce a significant increase in the tail DNA. Wound healing and zymography assays showed that GTN attenuated cell migration and caused a reduction in the activity level of two major migration-associated matrix metalloproteinases, MMP-2 and MMP-9. It can be concluded that the DNA-damaging effect of GTN against lung cancer cells leads to growth inhibition as well as a depression in migration ability. Therefore, GTN has potential as a chemotherapeutic agent against lung cancer.

AB - We evaluated the possible anticancer performance of a natural compound, goniothalamin (GTN), against human lung cancer using as a non-small cell lung cancer (NSCLC) cell line, H1299, as the model system. Cellular proliferation was significantly inhibited by GTN. Using an improved alkaline comet-nuclear extract (comet-NE) assay, GTN was found to induce a significant increase in the tail DNA. Wound healing and zymography assays showed that GTN attenuated cell migration and caused a reduction in the activity level of two major migration-associated matrix metalloproteinases, MMP-2 and MMP-9. It can be concluded that the DNA-damaging effect of GTN against lung cancer cells leads to growth inhibition as well as a depression in migration ability. Therefore, GTN has potential as a chemotherapeutic agent against lung cancer.

KW - Goniothalamin

KW - cell migration

KW - comet assay

KW - matrix metalloproteinase

KW - non-small cell lung cancer

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JO - Journal of Agricultural and Food Chemistry

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ER -

Goniothalamin inhibits growth of human lung cancer cells through DNA damage, apoptosis, and reduced migration ability

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UN SDG

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