Gold-Specific Biosensor for Monitoring Wastewater Using Genetically Engineered Cupriavidus metallidurans CH34

Ssu Tzu Tsai, Wen Jui Cheng, Qian Xian Zhang, Yi Chun Yeh*

*此作品的通信作者

研究成果: 雜誌貢獻期刊論文同行評審

摘要

Transcription factor-based whole-cell biosensors have recently become promising alternatives to conventional analytical methods due to their advantage of simplicity, cost-effectiveness, and environmental friendliness. In this study, we used genetic engineering to develop a whole-cell biosensor based on the activation of promoters by CupR via interactions with gold ions, leading to the expression of reporter genes that yield output signals. Altering the promoter sequences was shown to significantly improve the performance of the biosensor strain in terms of gold-specificity. The detection sensitivity of our engineered strains was 42-fold higher than that of wild-type strains. The linear range of the purposed sensor was 125–1000 nM with a limit of detection at 46.5 nM. The effectiveness of the sensor strain was verified in wastewater samples.

原文英語
頁(從 - 到)3576-3582
頁數7
期刊ACS Synthetic Biology
10
發行號12
DOIs
出版狀態已發佈 - 2021 12月 17

ASJC Scopus subject areas

  • 生物醫學工程
  • 生物化學、遺傳與分子生物學(雜項)

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