TY - JOUR
T1 - Glucose sensing based on an effective conversion of O2 and H2O2 into superoxide anion radical with clay minerals
AU - Chih, Tsailing
AU - Jao, Hsi Jung
AU - Wang, Chong Mou
N1 - Funding Information:
The authors thank the National Science Council, Republic of China (NSC 93-2113-M-003-019) for financial support.
PY - 2005/8/1
Y1 - 2005/8/1
N2 - An effective promotion for the generation of superoxide anion radical from H2O2 and O2 was accomplished with montmorillonite K10 clay mineral (denoted Mont. K10). This catalytic event was characterized by a fluorescence assay using amplex red and superoxide dismutase (SOD) as probes. The iron species (FeII and FeIII) contained in Mont. K10 are thought to contribute to this catalytic event. Due to this property, a glucose sensor was fabricated on the basis of Mont. K10, amplex red, and glucose oxidase (GOx). When glucose is injected with amplex red over the clay|GOx electrode, H2O2 is produced in the enzyme zone, transduced to superoxide anion radical by the clay, and quenched by amplex red. Since the resulting product, resorufin, is emission-active under the irradiation of visible light (λex: 563 nm; λem: 583 nm, pH 8), the analyte can thus be recognized and reported as optical signals with a luminescence spectrometer via a Y-type optical fiber atop of the electrode. The optical signal showed a linear response to glucose up to 150 μM at pH 8. The lower and upper limits were estimated to be 1.0 and 150 μM, respectively, at this pH. These results suggest that Mont. K10 is a promising O2- transducer.
AB - An effective promotion for the generation of superoxide anion radical from H2O2 and O2 was accomplished with montmorillonite K10 clay mineral (denoted Mont. K10). This catalytic event was characterized by a fluorescence assay using amplex red and superoxide dismutase (SOD) as probes. The iron species (FeII and FeIII) contained in Mont. K10 are thought to contribute to this catalytic event. Due to this property, a glucose sensor was fabricated on the basis of Mont. K10, amplex red, and glucose oxidase (GOx). When glucose is injected with amplex red over the clay|GOx electrode, H2O2 is produced in the enzyme zone, transduced to superoxide anion radical by the clay, and quenched by amplex red. Since the resulting product, resorufin, is emission-active under the irradiation of visible light (λex: 563 nm; λem: 583 nm, pH 8), the analyte can thus be recognized and reported as optical signals with a luminescence spectrometer via a Y-type optical fiber atop of the electrode. The optical signal showed a linear response to glucose up to 150 μM at pH 8. The lower and upper limits were estimated to be 1.0 and 150 μM, respectively, at this pH. These results suggest that Mont. K10 is a promising O2- transducer.
KW - Amplex red
KW - Clay
KW - Glucose
KW - Reactive oxygen species
KW - Superoxide anion radical
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U2 - 10.1016/j.jelechem.2004.12.018
DO - 10.1016/j.jelechem.2004.12.018
M3 - Article
AN - SCOPUS:21844440293
SN - 1572-6657
VL - 581
SP - 159
EP - 166
JO - Journal of Electroanalytical Chemistry
JF - Journal of Electroanalytical Chemistry
IS - 2
ER -