Globotriose-functionalized gold nanoparticles as multivalent probes for Shiga-like toxin

Yuh Yih Chien, Mi Dan Jan, Avijit Kumar Adak, Hsiao Chien Tzeng, Yen Ping Lin, Yu Ju Chen, Ken Tseng Wang, Chien Tien Chen*, Chia Chun Chen*, Chun Cheng Lin

*此作品的通信作者

研究成果: 雜誌貢獻期刊論文同行評審

97 引文 斯高帕斯(Scopus)

摘要

Compared to monovalent carbohydrates, multivalent carbohydrate ligands exhibit significantly enhanced binding affinities to their interacting proteins. Here, we report globotriose (Pk ligand)-functionalized gold nanoparticle (AuNP) probes for the investigation of multivalent interactions with the B5 subunit of Shiga-like toxin I (B-Slt). Six Pk-ligand-encapsulated AuNPs (Pk-AuNPs) of varying particle size and linker length were synthesized and evaluated for their potential as multivalent affinity probes by using a surface plasmon resonance competition assay. The affinity of these probes for the interacting proteins was greatly affected by nanoparticle size, linker length, and ligand density on nanoparticle surface. For example, the 20-nm 20-Pk-l-AuNP, which had a relatively long linker showed a >108-fold increase in affinity compared with the mono Pk ligand. This intrinsic high-affinity AuNP probe specifically captured the recombinant B-Slt from Escherichia coli lysate, and the resulting purity of the B-Slt was > 95 %. We also developed a robust Pk-AuNP-based detection method for Slt-I by combining the technique with silver enhancement.

原文英語
頁(從 - 到)1100-1109
頁數10
期刊ChemBioChem
9
發行號7
DOIs
出版狀態已發佈 - 2008 5月 5

ASJC Scopus subject areas

  • 生物化學
  • 分子醫學
  • 分子生物學
  • 有機化學

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