TY - JOUR
T1 - Gene expression in E. coli influences the position and motion of the lac operon and vicinal loci
AU - Lu, Yuan Chu
AU - Chang, Yi Ren
N1 - Publisher Copyright:
© 2019 Elsevier Inc.
PY - 2019/11/5
Y1 - 2019/11/5
N2 - Transcription and translation of active genes play an important role in determining the global organization of the chromosome. To further elucidate this phenomenon, we examined how the expression of either the lacY or the cfp gene in the native lac operon influences adjacent chromosomal segments by fluorescently labeling loci upstream and downstream of the expressed gene. Based on the positions and motile behaviors of these loci, our results reveal that the local organization of the vicinal chromosomal segments and its position in the nucleoid are both influenced by gene expression. Furthermore, we found that the effects on local organization depend on whether the expressed gene encodes a membrane protein or a cytoplasmic protein. Our measurements showing the movement of loci toward the membrane and the correlation between the motions of the upstream and downstream loci support the conclusion that the expression of genes encoding membrane proteins greatly influences chromosome dynamics.
AB - Transcription and translation of active genes play an important role in determining the global organization of the chromosome. To further elucidate this phenomenon, we examined how the expression of either the lacY or the cfp gene in the native lac operon influences adjacent chromosomal segments by fluorescently labeling loci upstream and downstream of the expressed gene. Based on the positions and motile behaviors of these loci, our results reveal that the local organization of the vicinal chromosomal segments and its position in the nucleoid are both influenced by gene expression. Furthermore, we found that the effects on local organization depend on whether the expressed gene encodes a membrane protein or a cytoplasmic protein. Our measurements showing the movement of loci toward the membrane and the correlation between the motions of the upstream and downstream loci support the conclusion that the expression of genes encoding membrane proteins greatly influences chromosome dynamics.
KW - DNA structure & dynamics
KW - Fluorescence super resolution imaging
KW - Single molecule methods
KW - Transcription
UR - http://www.scopus.com/inward/record.url?scp=85072012628&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85072012628&partnerID=8YFLogxK
U2 - 10.1016/j.bbrc.2019.09.026
DO - 10.1016/j.bbrc.2019.09.026
M3 - Article
C2 - 31522813
AN - SCOPUS:85072012628
SN - 0006-291X
VL - 519
SP - 438
EP - 443
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 2
ER -