Fragmented α-amylase into microporous metal-organic frameworks as bioreactors

Li Hao Liu, Ru Yin Chiu, Pamela Berilyn So, Stephen Lirio, Hsi Ya Huang, Wan Ling Liu, Chia Her Lin*

*此作品的通信作者

研究成果: 雜誌貢獻期刊論文同行評審

1 引文 斯高帕斯(Scopus)

摘要

This work presents an efficient and facile strategy to prepare an α-amylase bioreactor. As enzymes are quite large to be immobilized inside metal-organic frameworks (MOFs), the tertiary and quaternary structures of α-amylase were first disrupted using a combination of urea, dithio-threitol (DTT), and iodoacetamide (IAA). After losing its tertiary structure, the unfolded proteins can now penetrate into the microporous MOFs, affording fragmented α-amylase@MOF bioreactors. Among the different MOFs evaluated, UiO-66 gave the most promising potential due to the size-matching effect of the α-helix of the fragmented α-amylase with the pore size of UiO-66. The prepared bioreactor exhibited high yields of small carbohydrate (maltose) even when reused up to 15 times (>80% conversion).

原文英語
文章編號870
頁(從 - 到)1-8
頁數8
期刊Materials
14
發行號4
DOIs
出版狀態已發佈 - 2021 二月 2

ASJC Scopus subject areas

  • 材料科學(全部)

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