TY - JOUR
T1 - Forced expression of BCL-2 and BCL-xL by novel water-soluble fullerene, C60(glucosamine)6, reduces renal ischemia/reperfusion-induced oxidative stress
AU - Chien, Chiang Ting
AU - Chen, Chau Fong
AU - Hsu, Su Ming
AU - Chiang, Long Y.
AU - Lai, Ming Kuen
N1 - Funding Information:
This work was supported by the National Science Council of the Republic of China (NSC89-2314-B002-119 and NSC89-2314-B002-148) and partly by the National Taiwan University Hospital (NTUH89A014).
PY - 2001
Y1 - 2001
N2 - Apoptosis induced by oxidative stress, especially reactive oxygen species, resulting from ischemia/reperfusion would lead to organ dysfunction. C60(glucosamine)6, a water-soluble fullerene with strong free radical scavenging activity, was applied to evaluate its effect on renal ischemia/reperfusion-induced apoptosis formation and superoxide generation. C60(glucosamine)6 pretreatment, but not posttreatment, significantly reduced renal ischemia/reperfusion-induced apoptosis and superoxide generation and, consequently, ameliorated renal hemodynamic effects. Up-regulation in bcl-2 and bcl-xL of the rat kidney was evident in C60(glucosamine)6 pretreated, posttreated, and nontreated groups. However, exaggerated forced expression of bcl-2 and bcl-xL was found in the C60(glucosamine)6 pretreated group. Our results conclude that forced expression of bcl-2 and bcl-xL by the novel water-soluble fullerene, C60(glucosamine)6, can reduce renal ischemia/reperfusion-induced oxidative stress.
AB - Apoptosis induced by oxidative stress, especially reactive oxygen species, resulting from ischemia/reperfusion would lead to organ dysfunction. C60(glucosamine)6, a water-soluble fullerene with strong free radical scavenging activity, was applied to evaluate its effect on renal ischemia/reperfusion-induced apoptosis formation and superoxide generation. C60(glucosamine)6 pretreatment, but not posttreatment, significantly reduced renal ischemia/reperfusion-induced apoptosis and superoxide generation and, consequently, ameliorated renal hemodynamic effects. Up-regulation in bcl-2 and bcl-xL of the rat kidney was evident in C60(glucosamine)6 pretreated, posttreated, and nontreated groups. However, exaggerated forced expression of bcl-2 and bcl-xL was found in the C60(glucosamine)6 pretreated group. Our results conclude that forced expression of bcl-2 and bcl-xL by the novel water-soluble fullerene, C60(glucosamine)6, can reduce renal ischemia/reperfusion-induced oxidative stress.
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U2 - 10.1081/FST-100000167
DO - 10.1081/FST-100000167
M3 - Article
AN - SCOPUS:0034916354
SN - 1064-122X
VL - 9
SP - 77
EP - 88
JO - Fullerene Science and Technology
JF - Fullerene Science and Technology
IS - 1
ER -