Feasibility studies for assaying alpha-fetoprotein using antibody-activated magnetic nanoparticles

Kai Wen Huang, Shieh-Yueh Yang, Yu Wei Hong, Jen Jie Chieh, Che Chuan Yang, Herng-Er Horng*, Chau Chung Wu, Chin Yih Hong, Hong-Chang Yang

*此作品的通信作者

研究成果: 雜誌貢獻期刊論文同行評審

27 引文 斯高帕斯(Scopus)

摘要

Some previous reports have already shown the characterizations of immunomagnetic reduction (IMR). The assay technology involves the utilities of biofunctionalized magnetic nanoparticles to label target biomolecules. However, the detection threshold and interference tests for IMR have not been investigated in detail. In this study, alpha-fetoprotein (AFP) was used as a target biomolecule. The signals for AFP solutions of various concentrations, or with interfering materials, were detected via IMR. These samples were also used for characterizing the detection threshold and interference with enzyme-linked immunosorbent assay (ELISA). The results of assaying AFP level with IMR and ELISA were compared. The detection threshold for assaying AFP with IMR was found to be 3 ng/mL, which is 15 times lower than that of ELISA, and definitely suppresses false negative. For the interfering materials noted commonly in serum such as hemoglobin, bilirubin, triglyceride, and vascular endothelial growth factor, there was no detectable interfering effect when assaying AFP with IMR. Several serum samples from normal people and liver-tumor-bearing patients were used for the detections of AFP concentration via IMR. These results reveal the feasibilities of assaying AFP in blood using IMR, as well as achieving high-sensitive and high-specific assay for AFP.

原文英語
頁(從 - 到)1991-1996
頁數6
期刊International Journal of Nanomedicine
7
DOIs
出版狀態已發佈 - 2012

ASJC Scopus subject areas

  • 生物物理學
  • 生物工程
  • 生物材料
  • 藥學科學
  • 藥物發現
  • 有機化學

指紋

深入研究「Feasibility studies for assaying alpha-fetoprotein using antibody-activated magnetic nanoparticles」主題。共同形成了獨特的指紋。

引用此