Facilitation of conditioned fear extinction by D-cycloserine is mediated by mitogen-activated protein kinase and phosphatidylinositol 3-kinase cascades and requires de novo protein synthesis in basolateral nucleus of amygdala

Y. L. Yang, Kwok-Tung Lu*

*此作品的通信作者

研究成果: 雜誌貢獻期刊論文同行評審

89 引文 斯高帕斯(Scopus)

摘要

Recent results showed that either systemic or intra-amygdala administration of D-cycloserine, a partial agonist at the glycine modulatory site on the glutamate N-methyl-D-aspartate receptor facilitates the extinction of conditioned fear. Here we evaluated the role of mitogen-activated protein kinase and phosphatidylinositol 3-kinase in the basolateral nucleus of amygdala on the effect of D-cycloserine. The facilitation effect of D-cycloserine on fear extinction and mitogen-activated protein kinase activation was completely blocked by intra-amygdala administration of mitogen-activated protein kinase inhibitor PD98059 (500 ng/side, bilaterally) or U0-126 (20μM/side, bilaterally). Furthermore, phosphatidylinositol 3-kinase inhibitor (wortmannin, 5.0μg/side, bilaterally) infused into the basolateral nucleus of amygdala significantly reduced both facilitation effect of D-cycloserine and phosphatidylinositol 3-kinase activation. Intra-amygdala administration of a transcription inhibitor (actinomycin D, 10μg dissolved in 1.6μl vehicle; 0.8μl per side) and a translation inhibitor (anisomycin, 125μg dissolved in 1.6μl vehicle; 0.8μl per side) completely blocked the facilitation effect of D-cycloserine. Control experiments indicated the blockage by actinomycin D or anisomycin were not due to lasting damage to the basolateral nucleus of amygdala or state dependency. In addition, none of the active drugs used here altered the expression of conditioned fear. These results suggested that phosphatidylinositol 3-kinase and mitogen-activated protein kinase-dependent signaling cascades and new protein synthesis within the basolateral nucleus of amygdala played important roles in the D-cycloserine facilitation of the extinction of conditioned fear.

原文英語
頁(從 - 到)247-260
頁數14
期刊Neuroscience
134
發行號1
DOIs
出版狀態已發佈 - 2005

ASJC Scopus subject areas

  • 神經科學 (全部)

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