@article{bfb698373b5c48dfb7d7e80cf130141f,
title = "Extracts of Cerbera manghas L. effectively inhibit the viability of glioblastoma cell lines and their cancer stemloids in vitro and in mouse xenograft model",
abstract = "Glioblastoma multiforme (GBM), a highly recurrent malignant disease, is a grade IV glioma with poor prognosis. Glioblastoma stem cells (GSCs) are the new potential target for GBM intervention. To develop an improved treatment by targeting GSCs, we identified neriifolin, an active principle in extracts of Cerbera manghas L., as an effective inhibitor for the growth and migration of GBM cell lines and their tumorspheres. In this study, neriifolin effectively inhibited the growth and colony formation of CD133+ GBM tumorspheres. Both C. manghas extracts and neriifolin inhibited Akt activation and caused cell cycle G1 arrest. They also reduced the expression of the stem cell marker Sox2. Treatment with their higher doses induced the apoptosis of GBM tumorspheres. Furthermore, neriifolin inhibited the growth of CD133+ GBM tumorsphere-derived tumors in vivo. In summary, neriifolin obtained from extracts of C. manghas may serve as a drug lead compound for GBM therapies.",
keywords = "Cancer stem cell, Glioblastoma, Herbal extracts, Neriifolin",
author = "Tsai, {Jui Cheng} and Liu, {Wen Shan} and Tseng, {Ying Ting} and Lam, {Hoi Ian} and Chen, {Szu Ying} and Fang, {Cheng Li} and Tong, {Tien Soung} and Lai, {Yun Ju}",
note = "Funding Information: We would like to thank Dr. Ting-Shou Chen of the Industrial Technology Research Institute of Taiwan for providing the alcoholic extracts of C. manghas. We also thank Dr. Hsio-Mei Hsieh (National Taiwan Normal University) for providing the brains of newborn mice. We thank Taiwan Zebrafish CoreFacility at NHRI (Supported by MOST 107-2321-B001-045)) for providing Tg(kdrl:mCherry) fish for this study. This manuscript was edited by Dr. Louise T. Chow (University of Alabama at Birmingham) and Wallace Academic Editing. Funding Information: This work was supported by research grants from the Ministry of Science and Technology ( MOST 104-2320-B-003-010 and MOST 105-2320-B-003-007-MY3 ) and National Taiwan Normal University ( NTNU-103T3040B03 , NTNU-104T3040D3 , and NTNU-105T3040D3 ). Publisher Copyright: {\textcopyright} 2018 Elsevier Ltd",
year = "2018",
month = sep,
doi = "10.1016/j.jff.2018.07.017",
language = "English",
volume = "48",
pages = "283--296",
journal = "Journal of Functional Foods",
issn = "1756-4646",
publisher = "Elsevier Limited",
}
