Extracellular Ca2+ and Mg2+ modulate aminoglycoside blockade of mechanotransducer channel-mediated Ca2+ entry in zebrafish hair cells: An in vivo study with the SIET

Li Yih Lin, Wei Pang, Wei Min Chuang, Giun Yi Hung, Yuan Hsiang Lin, Jiun Lin Horng

研究成果: 雜誌貢獻文章

8 引文 (Scopus)

摘要

Zebrafish lateral-line hair cells are an in vivo model for studying hair cell development, function, and ototoxicity. However, the molecular identification and properties of the mechanotransducer (MET) channel in hair cells are still controversial. In this study, a noninvasive electrophysiological method, the scanning ion-electrode technique (SIET), was applied for the first time to investigate properties of MET channels in intact zebrafish embryos. With the use of a Ca2+-selective microelectrode to deflect hair bundles and simultaneously record the Ca2+ flux, the inward Ca2+ flux was detected at stereo cilia of hair cells in 2- to ~4-day postfer-tilization embryos. Ca2+ influx was blocked by MET channel blockers (BAPTA, La3+, Gd3+, and curare). In addition, 10 μM amino glycoside antibiotics (neomycin and gentamicin) were found to effectively block Ca2+ influx within 10 min. Elevating the external Ca2+ level (0.2-2 mM) neutralized the effects of neomycin and gentamicin. However, elevating the Mg2+ level up to 5 mM neutralized blockade by gentamicin but not by neomycin. This study demonstrated MET channel-mediated Ca2+ entry at hair cells and showed that the SIET to be a sensitive approach for functionally assaying MET channels in zebrafish.

原文英語
頁(從 - 到)C1060-C1068
期刊American Journal of Physiology - Cell Physiology
305
發行號10
DOIs
出版狀態已發佈 - 2013 十一月 15

指紋

Aminoglycosides
Zebrafish
Electrodes
Neomycin
Ions
Gentamicins
Embryonic Structures
Curare
Cilia
Microelectrodes
Glycosides
Anti-Bacterial Agents

ASJC Scopus subject areas

  • Physiology
  • Cell Biology

引用此文

Extracellular Ca2+ and Mg2+ modulate aminoglycoside blockade of mechanotransducer channel-mediated Ca2+ entry in zebrafish hair cells : An in vivo study with the SIET. / Lin, Li Yih; Pang, Wei; Chuang, Wei Min; Hung, Giun Yi; Lin, Yuan Hsiang; Horng, Jiun Lin.

於: American Journal of Physiology - Cell Physiology, 卷 305, 編號 10, 15.11.2013, p. C1060-C1068.

研究成果: 雜誌貢獻文章

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abstract = "Zebrafish lateral-line hair cells are an in vivo model for studying hair cell development, function, and ototoxicity. However, the molecular identification and properties of the mechanotransducer (MET) channel in hair cells are still controversial. In this study, a noninvasive electrophysiological method, the scanning ion-electrode technique (SIET), was applied for the first time to investigate properties of MET channels in intact zebrafish embryos. With the use of a Ca2+-selective microelectrode to deflect hair bundles and simultaneously record the Ca2+ flux, the inward Ca2+ flux was detected at stereo cilia of hair cells in 2- to ~4-day postfer-tilization embryos. Ca2+ influx was blocked by MET channel blockers (BAPTA, La3+, Gd3+, and curare). In addition, 10 μM amino glycoside antibiotics (neomycin and gentamicin) were found to effectively block Ca2+ influx within 10 min. Elevating the external Ca2+ level (0.2-2 mM) neutralized the effects of neomycin and gentamicin. However, elevating the Mg2+ level up to 5 mM neutralized blockade by gentamicin but not by neomycin. This study demonstrated MET channel-mediated Ca2+ entry at hair cells and showed that the SIET to be a sensitive approach for functionally assaying MET channels in zebrafish.",
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T1 - Extracellular Ca2+ and Mg2+ modulate aminoglycoside blockade of mechanotransducer channel-mediated Ca2+ entry in zebrafish hair cells

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AU - Pang, Wei

AU - Chuang, Wei Min

AU - Hung, Giun Yi

AU - Lin, Yuan Hsiang

AU - Horng, Jiun Lin

PY - 2013/11/15

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AB - Zebrafish lateral-line hair cells are an in vivo model for studying hair cell development, function, and ototoxicity. However, the molecular identification and properties of the mechanotransducer (MET) channel in hair cells are still controversial. In this study, a noninvasive electrophysiological method, the scanning ion-electrode technique (SIET), was applied for the first time to investigate properties of MET channels in intact zebrafish embryos. With the use of a Ca2+-selective microelectrode to deflect hair bundles and simultaneously record the Ca2+ flux, the inward Ca2+ flux was detected at stereo cilia of hair cells in 2- to ~4-day postfer-tilization embryos. Ca2+ influx was blocked by MET channel blockers (BAPTA, La3+, Gd3+, and curare). In addition, 10 μM amino glycoside antibiotics (neomycin and gentamicin) were found to effectively block Ca2+ influx within 10 min. Elevating the external Ca2+ level (0.2-2 mM) neutralized the effects of neomycin and gentamicin. However, elevating the Mg2+ level up to 5 mM neutralized blockade by gentamicin but not by neomycin. This study demonstrated MET channel-mediated Ca2+ entry at hair cells and showed that the SIET to be a sensitive approach for functionally assaying MET channels in zebrafish.

KW - Aminoglycoside

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KW - Neuromast

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