Exploration of the relationship between the tumor burden and the concentration of vascular endothelial growth factor in liver-cancer-bearing animals using immunomagnetic reduction assay

K. W. Huang, S. Y. Yang, C. Y. Yu, J. J. Chieh, Che Chuan Yang, Herng Er Horng, Chin Yih Hong, Hong Chang Yang, Chau Chung Wu

研究成果: 雜誌貢獻文章

39 引文 (Scopus)

摘要

To achieve early-stage diagnosis, a high-sensitivity assay method is needed. As a biomarker, vascular endothelial growth factor (VEGF) has played a growing role in diagnosing and treating hepatocellular carcinoma (HCC). In this work, an immunomagnetic reduction (IMR) through bio-functionalized magnetic nanoparticles and a high-temperature superconducting-quantum-interference-device magnetometer were utilized for quantitative detection of low-concentration VEGF in serum from rats with HCC. The precision and accuracy of IMR on VEGF were characterized. Further, the results of assaying VEGF in the serum of rats were compared with those of using enzyme-linked immunosorbant assay (ELISA). It was found the correlations between the detected VEGF concentration in the rat serum and tumor burdens were 0.99 and 0.90 for IMR and ELISA, respectively, within the range from 2 pg/ml to 8000 pg/ml of VEGF concentration.

原文英語
頁(從 - 到)535-541
頁數7
期刊Journal of Biomedical Nanotechnology
7
發行號4
DOIs
出版狀態已發佈 - 2011 八月 1

指紋

Bearings (structural)
Liver Neoplasms
Tumor Burden
Liver
Vascular Endothelial Growth Factor A
Tumors
Assays
Animals
Rats
Hepatocellular Carcinoma
Enzymes
Serum
SQUIDs
Biomarkers
Magnetometers
Nanoparticles
Intercellular Signaling Peptides and Proteins
Early Diagnosis
Equipment and Supplies
Temperature

ASJC Scopus subject areas

  • Bioengineering
  • Medicine (miscellaneous)
  • Biomedical Engineering
  • Materials Science(all)
  • Pharmaceutical Science

引用此文

Exploration of the relationship between the tumor burden and the concentration of vascular endothelial growth factor in liver-cancer-bearing animals using immunomagnetic reduction assay. / Huang, K. W.; Yang, S. Y.; Yu, C. Y.; Chieh, J. J.; Yang, Che Chuan; Horng, Herng Er; Hong, Chin Yih; Yang, Hong Chang; Wu, Chau Chung.

於: Journal of Biomedical Nanotechnology, 卷 7, 編號 4, 01.08.2011, p. 535-541.

研究成果: 雜誌貢獻文章

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abstract = "To achieve early-stage diagnosis, a high-sensitivity assay method is needed. As a biomarker, vascular endothelial growth factor (VEGF) has played a growing role in diagnosing and treating hepatocellular carcinoma (HCC). In this work, an immunomagnetic reduction (IMR) through bio-functionalized magnetic nanoparticles and a high-temperature superconducting-quantum-interference-device magnetometer were utilized for quantitative detection of low-concentration VEGF in serum from rats with HCC. The precision and accuracy of IMR on VEGF were characterized. Further, the results of assaying VEGF in the serum of rats were compared with those of using enzyme-linked immunosorbant assay (ELISA). It was found the correlations between the detected VEGF concentration in the rat serum and tumor burdens were 0.99 and 0.90 for IMR and ELISA, respectively, within the range from 2 pg/ml to 8000 pg/ml of VEGF concentration.",
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AU - Yang, S. Y.

AU - Yu, C. Y.

AU - Chieh, J. J.

AU - Yang, Che Chuan

AU - Horng, Herng Er

AU - Hong, Chin Yih

AU - Yang, Hong Chang

AU - Wu, Chau Chung

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AB - To achieve early-stage diagnosis, a high-sensitivity assay method is needed. As a biomarker, vascular endothelial growth factor (VEGF) has played a growing role in diagnosing and treating hepatocellular carcinoma (HCC). In this work, an immunomagnetic reduction (IMR) through bio-functionalized magnetic nanoparticles and a high-temperature superconducting-quantum-interference-device magnetometer were utilized for quantitative detection of low-concentration VEGF in serum from rats with HCC. The precision and accuracy of IMR on VEGF were characterized. Further, the results of assaying VEGF in the serum of rats were compared with those of using enzyme-linked immunosorbant assay (ELISA). It was found the correlations between the detected VEGF concentration in the rat serum and tumor burdens were 0.99 and 0.90 for IMR and ELISA, respectively, within the range from 2 pg/ml to 8000 pg/ml of VEGF concentration.

KW - Animal model

KW - Hepatocellular carcinoma

KW - Immunomagnetic reduction

KW - Magnetic nanoparticle

KW - Vascular endothelial growth factor

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