Exploration of the relationship between the tumor burden and the concentration of vascular endothelial growth factor in liver-cancer-bearing animals using immunomagnetic reduction assay

K. W. Huang, S. Y. Yang, C. Y. Yu, J. J. Chieh, Che Chuan Yang, Herng Er Horng*, Chin Yih Hong, Hong Chang Yang, Chau Chung Wu

*此作品的通信作者

    研究成果: 雜誌貢獻期刊論文同行評審

    42 引文 斯高帕斯(Scopus)

    摘要

    To achieve early-stage diagnosis, a high-sensitivity assay method is needed. As a biomarker, vascular endothelial growth factor (VEGF) has played a growing role in diagnosing and treating hepatocellular carcinoma (HCC). In this work, an immunomagnetic reduction (IMR) through bio-functionalized magnetic nanoparticles and a high-temperature superconducting-quantum-interference-device magnetometer were utilized for quantitative detection of low-concentration VEGF in serum from rats with HCC. The precision and accuracy of IMR on VEGF were characterized. Further, the results of assaying VEGF in the serum of rats were compared with those of using enzyme-linked immunosorbant assay (ELISA). It was found the correlations between the detected VEGF concentration in the rat serum and tumor burdens were 0.99 and 0.90 for IMR and ELISA, respectively, within the range from 2 pg/ml to 8000 pg/ml of VEGF concentration.

    原文英語
    頁(從 - 到)535-541
    頁數7
    期刊Journal of Biomedical Nanotechnology
    7
    發行號4
    DOIs
    出版狀態已發佈 - 2011 八月 1

    ASJC Scopus subject areas

    • 生物工程
    • 醫藥(雜項)
    • 生物醫學工程
    • 材料科學(全部)
    • 藥學科學

    指紋

    深入研究「Exploration of the relationship between the tumor burden and the concentration of vascular endothelial growth factor in liver-cancer-bearing animals using immunomagnetic reduction assay」主題。共同形成了獨特的指紋。

    引用此