Experimental study on low-detection limit for immunomagnetic reduction assays by manipulating reagent entities

Shieh-Yueh Yang, C. C. Yang, Herng-Er Horng*, B. Y. Shih, J. J. Chieh, C. Y. Hong, Hong-Chang Yang

*此作品的通信作者

研究成果: 雜誌貢獻期刊論文同行評審

11 引文 斯高帕斯(Scopus)

摘要

The low limit of detection (LLD) plays an important role in biomolecular assays, especially for early-stage assays. Biomolecular detections usually involve the use of two main elements: a reagent and an analyzer, which both greatly contribute to the LLD. In this work, the relationships among the LLD and reagent-related factors are investigated. The to-be-detected biomolecule is c-reactive protein (CRP) as an example. The assay method is immunomagnetic reduction (IMR). The components of reagent are ${\rm Fe}-{3}{\rm O} 4 magnetic nanoparticles bio-functionalized with antibodies against CRP, dispersed in pH-7.4 phosphate buffered saline solution. Several key factors of the reagent, such as particle concentration, volume ratio of reagent to sample, and particle size, are manipulated to optimize the LLD of detecting CRP.

原文英語
文章編號6451290
頁(從 - 到)65-68
頁數4
期刊IEEE Transactions on Nanobioscience
12
發行號2
DOIs
出版狀態已發佈 - 2013

ASJC Scopus subject areas

  • 生物技術
  • 生物工程
  • 醫藥(雜項)
  • 生物醫學工程
  • 藥學科學
  • 電腦科學應用
  • 電氣與電子工程

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