TY - JOUR
T1 - Experimental study on low-detection limit for immunomagnetic reduction assays by manipulating reagent entities
AU - Yang, Shieh-Yueh
AU - Yang, C. C.
AU - Horng, Herng-Er
AU - Shih, B. Y.
AU - Chieh, J. J.
AU - Hong, C. Y.
AU - Yang, Hong-Chang
PY - 2013
Y1 - 2013
N2 - The low limit of detection (LLD) plays an important role in biomolecular assays, especially for early-stage assays. Biomolecular detections usually involve the use of two main elements: a reagent and an analyzer, which both greatly contribute to the LLD. In this work, the relationships among the LLD and reagent-related factors are investigated. The to-be-detected biomolecule is c-reactive protein (CRP) as an example. The assay method is immunomagnetic reduction (IMR). The components of reagent are ${\rm Fe}-{3}{\rm O} 4 magnetic nanoparticles bio-functionalized with antibodies against CRP, dispersed in pH-7.4 phosphate buffered saline solution. Several key factors of the reagent, such as particle concentration, volume ratio of reagent to sample, and particle size, are manipulated to optimize the LLD of detecting CRP.
AB - The low limit of detection (LLD) plays an important role in biomolecular assays, especially for early-stage assays. Biomolecular detections usually involve the use of two main elements: a reagent and an analyzer, which both greatly contribute to the LLD. In this work, the relationships among the LLD and reagent-related factors are investigated. The to-be-detected biomolecule is c-reactive protein (CRP) as an example. The assay method is immunomagnetic reduction (IMR). The components of reagent are ${\rm Fe}-{3}{\rm O} 4 magnetic nanoparticles bio-functionalized with antibodies against CRP, dispersed in pH-7.4 phosphate buffered saline solution. Several key factors of the reagent, such as particle concentration, volume ratio of reagent to sample, and particle size, are manipulated to optimize the LLD of detecting CRP.
KW - Immunomagnetic reduction
KW - magnetic nanoparticles
KW - reagent
UR - http://www.scopus.com/inward/record.url?scp=84878678767&partnerID=8YFLogxK
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U2 - 10.1109/TNB.2013.2240009
DO - 10.1109/TNB.2013.2240009
M3 - Article
C2 - 23392387
AN - SCOPUS:84878678767
SN - 1536-1241
VL - 12
SP - 65
EP - 68
JO - IEEE Transactions on Nanobioscience
JF - IEEE Transactions on Nanobioscience
IS - 2
M1 - 6451290
ER -