TY - JOUR
T1 - Ethanolic extract of origanum vulgare suppresses propionibacterium acnes-induced inflammatory responses in human monocyte and mouse ear edema models
AU - Chuang, Lu Te
AU - Tsai, Tsung Hsien
AU - Lien, Tsung Jung
AU - Huang, Wen Cheng
AU - Liu, Jun Jen
AU - Chang, Hsiang
AU - Chang, Mei Ling
AU - Tsai, Po Jung
N1 - Publisher Copyright:
© 2018 MDPI AG. All rights reserved.
PY - 2018
Y1 - 2018
N2 - Acne vulgaris (acne) is a common inflammatory skin disorder, and Propionibacterium acnes plays a major role in the development and progression of acne inflammation. Herbs possessing antimicrobial and anti-inflammatory activity have been applied as a medical option for centuries. In this study, we examined the suppressive effect of ethanolic oregano (Origanum vulgare) extract on live P. Acnes-induced in vivo and in vitro inflammation. Following ethanol extraction of oregano leaves, four compounds with strong antioxidant activity, including rosmarinic acid, quercetin, apigenin, and carvacrol, were identified by high-performance liquid chromatography. Using the mouse ear edema model, we demonstrated that ethanol oregano extracts (EOE) significantly suppressed P. Acnes-induced skin inflammation, as measured by ear thickness (32%) and biopsy weight (37%). In a separate study, using the co-culture of P. Acnes and human THP-1 monocytes, EOE reduced the production of interleukin (IL)-8, IL-1κand tumor necrosis factor (TNF)-κup to 40%, 37%, and 18%, respectively, as well as the expression of these three pro-inflammatory mediators at the transcriptional level. Furthermore, EOE inhibited the translocation of nuclear factor-kappa B (NF-κB) into the nucleus possibly by inactivating toll-like receptor-2 (TLR2). The suppressive effect of EOE on live P. Acnes-induced inflammatory responses could be due, in part, to the anti-inflammatory and antioxidant properties, but not the anti-microbial effect of EOE.
AB - Acne vulgaris (acne) is a common inflammatory skin disorder, and Propionibacterium acnes plays a major role in the development and progression of acne inflammation. Herbs possessing antimicrobial and anti-inflammatory activity have been applied as a medical option for centuries. In this study, we examined the suppressive effect of ethanolic oregano (Origanum vulgare) extract on live P. Acnes-induced in vivo and in vitro inflammation. Following ethanol extraction of oregano leaves, four compounds with strong antioxidant activity, including rosmarinic acid, quercetin, apigenin, and carvacrol, were identified by high-performance liquid chromatography. Using the mouse ear edema model, we demonstrated that ethanol oregano extracts (EOE) significantly suppressed P. Acnes-induced skin inflammation, as measured by ear thickness (32%) and biopsy weight (37%). In a separate study, using the co-culture of P. Acnes and human THP-1 monocytes, EOE reduced the production of interleukin (IL)-8, IL-1κand tumor necrosis factor (TNF)-κup to 40%, 37%, and 18%, respectively, as well as the expression of these three pro-inflammatory mediators at the transcriptional level. Furthermore, EOE inhibited the translocation of nuclear factor-kappa B (NF-κB) into the nucleus possibly by inactivating toll-like receptor-2 (TLR2). The suppressive effect of EOE on live P. Acnes-induced inflammatory responses could be due, in part, to the anti-inflammatory and antioxidant properties, but not the anti-microbial effect of EOE.
KW - Anti-inflammatory
KW - NF-αB
KW - Oregano
KW - Propionibacterium acnes
KW - TLR2
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U2 - 10.3390/molecules23081987
DO - 10.3390/molecules23081987
M3 - Article
C2 - 30096960
AN - SCOPUS:85053689774
SN - 1420-3049
VL - 23
JO - Molecules
JF - Molecules
IS - 8
M1 - 1987
ER -