Endothelin-1 regulates hh +-atpase-dependent transepithelial hh + secretion in zebrafish

Ying Jey Guh, Yung Che Tseng, Chao Yew Yang, Pung Pung Hwang*

*此作品的通信作者

研究成果: 雜誌貢獻期刊論文同行評審

12 引文 斯高帕斯(Scopus)

摘要

Endothelin-1 (EDN1) is an important regulator of H + secretion in the mammalian kidney. EDN1 enhances renal tubule H +-ATPase activity, but the underlying mechanism remains unclear. To further elucidate the role of EDN1 in vertebrates' acid-base regulation, the present study used zebrafish as the model to examine the effects of EDN1 and its receptors on transepithelial H + secretion. Expression of EDN1 and one of its receptors, EDNRAa, was stimulated in zebrafish acclimated to acidic water. A noninvasive scanning ion-selective electrode technique was used toshowthat edn1 overexpression enhancesH +secretion in embryonic skin at 3 days post fertilization.EDNRAa loss of function significantly decreased EDN1- and acid-induced H + secretion. Abrogationof EDN1-enhanced H + secretion by a vacuolar H +-ATPase inhibitor (bafilomycin A1) suggests thatEDN1 exerts its action by regulating the H +-ATPase- mediated H + secretion. EDN1 does not appearto affect H + secretion through either altering the abundance of H +-ATPase or affecting the celldifferentiation of H +-ATPase-rich ionocytes, because the reduction in secretion upon ednraaknockdown was not accompanied by decreased expression of H +-ATPase or reduced H +- ATPaserichcell density. These findings provide evidence that EDN1 signaling is involved in acid-baseregulation in zebrafish and enhance our understanding of EDN1 regulation of transepithelial H +secretion in vertebrates.

原文英語
頁(從 - 到)1728-1737
頁數10
期刊Endocrinology
155
發行號5
DOIs
出版狀態已發佈 - 2014 五月

ASJC Scopus subject areas

  • 內分泌

指紋

深入研究「Endothelin-1 regulates hh <sup>+</sup>-atpase-dependent transepithelial hh <sup>+</sup> secretion in zebrafish」主題。共同形成了獨特的指紋。

引用此