Encephalomyocarditis virus is potentially derived from eastern bent-wing bats living in East Asian countries

Karla Cristine C. Doysabas, Mami Oba, Masaya Furuta, Keisuke Iida, Tsutomu Omatsu, Tetsuya Furuya, Takashi Okada, Kripitch Sutummaporn, Hiroshi Shimoda, Min Liang Wong, Chung Hsin Wu, Yasushige Ohmori, Ryosuke Kobayashi, Yupadee Hengjan, Kenzo Yonemitsu, Ryusei Kuwata, Yoo Kyung Kim, Sang Hyun Han, Joon Hyuk Sohn, Sang Hoon HanKazuo Suzuki, Junpei Kimura, Ken Maeda, Hong Shik Oh, Daiji Endoh, Tetsuya Mizutani, Eiichi Hondo*

*此作品的通信作者

研究成果: 雜誌貢獻期刊論文同行評審

3 引文 斯高帕斯(Scopus)

摘要

Bats are reservoir hosts of many zoonotic viruses and identification of viruses that they carry is important. This study aimed to use high throughput screening to identify the viruses in fecal guano of Taiwanese insectivorous bats caves in order to obtain more information on bat-derived pathogenic viruses in East Asia. Guano samples were collected from two caves in Taiwan, pooled, and then subjected to Multiplex PCR-based next generation sequencing for viral identification. Subsequently, encephalomyocarditis virus (EMCV) sequence was detected and confirmed by reverse transcription PCR. EMCV is considered as rodent virus and thus, animal species identification through cytochrome oxidase I (COI) barcoding was further done to identify the viral source. Finally, determination of distribution and verification of the presence of EMCV in guano obtained from Japanese and South Korean caves was also done. We concluded that the guano collected was not contaminated with the excrement of rodents which were reported and presumed to live in Taiwan. Also, EMCV genome fragments were found in guanos of Japanese and South Korean caves. It is possible that the eastern bent-wing bat (Miniopterus fuliginosus) is one of the natural hosts of EMCV in East Asia.

原文英語
頁(從 - 到)62-67
頁數6
期刊Virus Research
259
DOIs
出版狀態已發佈 - 2019 一月 2

ASJC Scopus subject areas

  • 癌症研究
  • 病毒學
  • 傳染性疾病

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