Electrochemical characterization of the clay-enhanced luminol ecl reaction

Chih Sheng Ouyang, Chong Mou Wang

研究成果: 雜誌貢獻期刊論文同行評審

3 引文 斯高帕斯(Scopus)

摘要

Clay modified electrodes were prepared with iron-containing clay particles (montmorillonite K10) and characterized for their ability to promote electrogenerated chemiluminescence (ecl) in a solution containing luminol and hydrogen peroxide. Electrochemical impedance spectroscopic (EIS) measurements showed that the charge-transfer resistance measured for the clay electrode decreased systematically with the addition of luminol or H2O2, leading to a linear relationship between the exchange current and the concentration of H2O2 or luminol in the region where [luminol]/[H2O2] or [H2O2]/[luminol] < 1. Analysis of the parallel conductance and capacitance showed that the iron species contained in the clay particles behaved as an efficient electron mediator for the luminol ecl reaction and the content of the iron species was roughly 40 ± 20 mg of clay. This clay-enhanced reaction showed potential for the detection of glucose after further modification with glucose oxidase (GOx). However, a gradual decay in the ecl signal was noted when the luminol was not immobilized. By using aminopropyltrimethoxysilane as an anchoring agent, a rapid and stable ecl response to glucose resulted and persisted for several minutes. Further attempts to enhance the electrode response to glucose were made by using fluorescein as an energy transfer mediator. The quantum efficiency of this energy-transfer reaction was about 1%. This low yield was tentatively ascribed to a light scattering or absorption by the enzyme layer.

原文英語
頁(從 - 到)82-88
頁數7
期刊Journal of Electroanalytical Chemistry
474
發行號1
DOIs
出版狀態已發佈 - 1999 9月 16

ASJC Scopus subject areas

  • 分析化學
  • 化學工程 (全部)
  • 電化學

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