Electrical detection of protein using gold nanoparticles and nanogap electrodes

Chien Ying Tsai, Tien Li Chang, Ramesh Uppala, Chun Chi Chen, K. O. Fu-Hsiang, Ping Hei Chen

研究成果: 雜誌貢獻文章同行評審

6 引文 斯高帕斯(Scopus)


A method of electrically detecting of protein described is developed using self-assembled multilayer gold nanoparticles (AuNPs) on a SiO2/Si substrate between gold electrodes. Electrical measurements are performed at room temperature using a probe station. A monoclonal antibody is immobilized on the top surface of the first layer of AuNPs (14nm). The second layer of AuNPs is formed through specific binding among a target antigen [hepatitis C virus, (HCV)], the monoclonal antibody, and the conjugate of a AuNP-polyclonal antibody. Once the specific binding among the monoclonal antibody, target antigen, and polyclonal antibody occurs, a significant electric current is detected through multilayer self-assembled gold nanoparticles between nanogap electrodes. No significant current (<1 pA) can be measured through a monolayer of AuNPs. A significant difference between the IV curves of the monolayer and the multilayer of AuNPs is used to identify whether the target antigen exists in the tested sample.

頁(從 - 到)5711-5716
期刊Japanese Journal of Applied Physics, Part 1: Regular Papers and Short Notes and Review Papers
發行號7 B
出版狀態已發佈 - 2005 七月 26

ASJC Scopus subject areas

  • Engineering(all)
  • Physics and Astronomy(all)

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