Effects of stanniocalcin 1 on calcium uptake in zebrafish (Danio rerio) embryo

Deng Yu Tseng, Ming Yi Chou, Yung Che Tseng, Chung Der Hsiao, Chang Jen Huang, Toyoji Kaneko, Pung Pung Hwang

研究成果: 雜誌貢獻文章

70 引文 斯高帕斯(Scopus)

摘要

Stanniocalcin (STC) formerly called hypocalcin or teleocalcin, is a 50-kDa disulfide-linked homodimeric glycoprotein that was originally identified in fish and secreted from the corpuscles of Stannius (CS). One of the main functions of STC-1 is Ca2+ uptake inhibition; however, the mechanisms remain unknown. In the present study, we provide molecular evidence to elucidate how zebrafish STC-1 regulates Ca2+ uptake in zebrafish embryos. In a wide variety of tissues including the kidney, brain, gill, muscle, and skin, zstc-1 was expressed. Incubating zebrafish embryos in low-Ca2+ (0.02 mM) freshwater stimulated whole body Ca2+ influx and zebrafish epithelial Ca2+ channel (zECaC) mRNA expression, while downregulated zstc-1 expression. A morpholino microinjection approach was used to knockdown the zSTC-1 protein, and the results showed that the Ca2+ content, Ca 2+ influx, and zECaC mRNA expression all increased in morphants. These data suggest that zSTC-1 negatively regulates ECaC gene expression to reduce Ca2+ uptake in zebrafish embryos.

原文英語
頁(從 - 到)R549-R557
期刊American Journal of Physiology - Regulatory Integrative and Comparative Physiology
296
發行號3
DOIs
出版狀態已發佈 - 2009 三月 1

ASJC Scopus subject areas

  • Physiology
  • Physiology (medical)

指紋 深入研究「Effects of stanniocalcin 1 on calcium uptake in zebrafish (Danio rerio) embryo」主題。共同形成了獨特的指紋。

  • 引用此