Effects of stanniocalcin 1 on calcium uptake in zebrafish (Danio rerio) embryo

Deng Yu Tseng, Ming Yi Chou, Yung Che Tseng, Chung Der Hsiao, Chang Jen Huang, Toyoji Kaneko, Pung Pung Hwang*


研究成果: 雜誌貢獻期刊論文同行評審

75 引文 斯高帕斯(Scopus)


Stanniocalcin (STC) formerly called hypocalcin or teleocalcin, is a 50-kDa disulfide-linked homodimeric glycoprotein that was originally identified in fish and secreted from the corpuscles of Stannius (CS). One of the main functions of STC-1 is Ca2+ uptake inhibition; however, the mechanisms remain unknown. In the present study, we provide molecular evidence to elucidate how zebrafish STC-1 regulates Ca2+ uptake in zebrafish embryos. In a wide variety of tissues including the kidney, brain, gill, muscle, and skin, zstc-1 was expressed. Incubating zebrafish embryos in low-Ca2+ (0.02 mM) freshwater stimulated whole body Ca2+ influx and zebrafish epithelial Ca2+ channel (zECaC) mRNA expression, while downregulated zstc-1 expression. A morpholino microinjection approach was used to knockdown the zSTC-1 protein, and the results showed that the Ca2+ content, Ca 2+ influx, and zECaC mRNA expression all increased in morphants. These data suggest that zSTC-1 negatively regulates ECaC gene expression to reduce Ca2+ uptake in zebrafish embryos.

頁(從 - 到)R549-R557
期刊American Journal of Physiology - Regulatory Integrative and Comparative Physiology
出版狀態已發佈 - 2009 三月

ASJC Scopus subject areas

  • 生理學
  • 生理學(醫學)


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