TY - JOUR
T1 - Dual-Signal Microbial Biosensor for the Detection of Dopamine without Inference from Other Catecholamine Neurotransmitters
AU - Lin, Yu Kuan
AU - Yeh, Yi Chun
N1 - Publisher Copyright:
© 2017 American Chemical Society.
PY - 2017/11/7
Y1 - 2017/11/7
N2 - Dopamine, one of catecholamine neurotransmitters, plays an important role in many brain functions and behavioral responses. In this study, we developed a novel dual-signal whole-cell biosensor for the detection of dopamine through the generation of red fluorescent proteins and 6-decarboxylated betaxanthin pigments. The proposed system responses specifically to dopamine with a detection limit of 1.43 μM. Furthermore, a combination of dual output signals makes it possible to reduce the interference from other catecholamine neurotransmitters, including L-DOPA, epinephrine, and norepinephrine.
AB - Dopamine, one of catecholamine neurotransmitters, plays an important role in many brain functions and behavioral responses. In this study, we developed a novel dual-signal whole-cell biosensor for the detection of dopamine through the generation of red fluorescent proteins and 6-decarboxylated betaxanthin pigments. The proposed system responses specifically to dopamine with a detection limit of 1.43 μM. Furthermore, a combination of dual output signals makes it possible to reduce the interference from other catecholamine neurotransmitters, including L-DOPA, epinephrine, and norepinephrine.
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U2 - 10.1021/acs.analchem.7b02498
DO - 10.1021/acs.analchem.7b02498
M3 - Article
C2 - 29027462
AN - SCOPUS:85045907251
SN - 0003-2700
VL - 89
SP - 11178
EP - 11182
JO - Analytical Chemistry
JF - Analytical Chemistry
IS - 21
ER -