DNA-gold nanorod conjugates for remote control of localized gene expression by near infrared irradiation

Chia Chun Chen, Yen Ping Lin, Chih Wei Wang, Hsiao Chien Tzeng, Chia Hsuan Wu, Yi Cheng Chen, Chin Pei Chen, Li Chyong Chen, Yi Chun Wu

研究成果: 雜誌貢獻期刊論文同行評審

379 引文 斯高帕斯(Scopus)

摘要

Gold nanorods were attached to the gene of enhanced green fluorescence protein (EGFP) for the remote control of gene expression in living cells. The UV-vis spectroscopy, electrophoresis, and transmission electron microscopy (TEM) were used to study the optical and structural properties of the EGFP DNA and gold nanorod (EGFP-GNR) conjugates before and after femto-second near-infrared (NIR) laser irradiation. Upon NIR irradiation, the gold nanorods of EGFP-GNR conjugates underwent shape transformation that resulted in the release of EGFP DNA. When EGFP-GNR conjugates were delivered to cultured HeLa cells, induced GFP expression was specifically observed in cells that were locally exposed to NIR irradiation. Our results demonstrate the feasibility of using gold nanorods and NIR irradiation as means of remote control of gene expression in specific cells. This approach has potential applications in biological and medical studies.

原文英語
頁(從 - 到)3709-3715
頁數7
期刊Journal of the American Chemical Society
128
發行號11
DOIs
出版狀態已發佈 - 2006 三月 22

ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)
  • Biochemistry
  • Colloid and Surface Chemistry

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