Development of a whole-cell biosensor for the determination of tyrosine in urine for point-of-care diagnostics

Chieh Lin, Qian Xian Zhang, Yi Chun Yeh

研究成果: 雜誌貢獻文章

2 引文 斯高帕斯(Scopus)

摘要

Most disorders associated with tyrosine metabolism lead to elevated tyrosine levels in biological fluids. In this work, we used genetic engineering techniques to develop a simple and low-cost biosensor for tyrosine quantification. Here, two tyrosine-responsive promoters are used to differentially control the production of red and green fluorescent proteins signals in response to tyrosine levels in a culture. We also conducted a systematic examination of various modular components (promoters and ribosome binding sites) in order to ensure that our biosensor was capable of rapidly producing a pronounced fluorescence signal. To make the device more user-friendly, we developed a reagentless platform in which biocompatible agarose is used as an entrapment agent with cell sensors and growth media mounted within the gel matrix.

原文英語
頁(從 - 到)1400-1404
頁數5
期刊Analytical Methods
11
發行號10
DOIs
出版狀態已發佈 - 2019 三月 14

ASJC Scopus subject areas

  • Analytical Chemistry
  • Chemical Engineering(all)
  • Engineering(all)

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