Development of a subunit vaccine containing recombinant chicken anemia virus VP1 and pigeon IFN-γ

Sin Ying Shen, Wei Chun Chang, Hsiang Heng Yi, Shinn Shong Tsai, Hung Jen Liu, Pei Chun Liao, Kuo Pin Chuang*

*此作品的通信作者

研究成果: 雜誌貢獻期刊論文同行評審

7 引文 斯高帕斯(Scopus)

摘要

Chicken anemia virus (CAV) is a severe threat to the chicken industry and causes heavy economic losses worldwide. In this study, we evaluated the immune response and protective efficacy provided by a subunit vaccine containing recombinant VP1 (rVP1) and pigeon interferon-γ (rPiIFN-γ). Results indicated that rPiIFN-γ enhanced humoral immunity elicited by rVP1 as early as 10 day after primary immunization and reach the high titer after secondary immunization. When compared to chickens immunized with rVP1, inactivated vaccine, chickens immunized with rVP1. +. rPiIFN-γ showed faster and higher levels (. p<. 0.05) of antibody titer. The CAV challenge result showed that the rVP1. +. rPiIFN-γ vaccine prevent the reducing of hematocrit values in comparison with the rVP1 or inactivated groups. The relative fold inductions of mRNA expression of Th1-type (IFN-γ), but not Th2-type (IL-4) cytokines in splenocytes isolated from chickens immunized with rVP1. +. rPiIFN-γ were significantly higher than those of the rVP1 or inactivated vaccine groups. In conclusion, our study found that rPiIFN-γ can enhance both humoral and cellular immunity elicited by an rVP1 vaccine. The rVP1. +. rPiIFN-γ vaccine may provide a new strategy vaccine against CAV in chicken.

原文英語
頁(從 - 到)200-204
頁數5
期刊Veterinary Immunology and Immunopathology
167
發行號3-4
DOIs
出版狀態已發佈 - 2015 十月 15

ASJC Scopus subject areas

  • 免疫學
  • 獸醫 (全部)

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