This study tries to develop an animation-based test (ABT) in the area of Earth science. The advantages of ABT includes: (1) To present more authentic situation in an animated testing environment; (2) To assess the learning outcomes with appropriate validity and reliability; (3) To be a more attractive way of testing. The content of the test focuses on four domains in Earth science including astronomy, meteorology, oceanography and geology. "Attitude toward Animated Assessment Scale" (AAAS) was adapted in this study in order to explore examinees' perceptions and attitudes toward ABT. This study found the ABT to be generally validate and reliable and test-takers had better performance in the animation-based testing environment.