Development and characterization of novel microsatellite markers in chestnut tiger butterfly Parantica sita (Lepidoptera: Nymphalidae) using next-generation sequencing

Ping Hu, Chia Lung Huang, Min Xin Luo, Yu Feng Hsu, Rong Jiang Wang

研究成果: 雜誌貢獻文章

1 引文 斯高帕斯(Scopus)

摘要

While one subspecies of chestnut tiger butterfly (Parantica sita, Lepitoptera: Nymphalidae) undertakes seasonal migration between Taiwan and Japan, it is still unknown whether the nominate subspecies, P. s. sita, migrates seasonally in southwest China. To understand the potential migration of P. s. sita, it is necessary to investigate the genetic differentiation among populations. In our study, we developed 18 novel microsatellite markers from the transcriptome using next-generation sequencing methods. These polymorphic markers were tested on 89 individuals from 4 populations of P. sita. The allele numbers ranged from 2 to 19. The average polymorphism information content values in each population ranged from 0.34 to 0.40. The observed (Ho) and expected (He) heterozygosity of each locus in each population ranged from 0.043 to 0.870 and 0.043 to 0.833, respectively. These informative microsatellite markers would be helpful for discovering the facts of P. sita long-distance migration in China.

原文英語
頁(從 - 到)281-286
頁數6
期刊Applied Entomology and Zoology
55
發行號2
DOIs
出版狀態已發佈 - 2020 五月 1
對外發佈Yes

ASJC Scopus subject areas

  • Insect Science

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