Determination of Gold Ions in Human Urine Using Genetically Engineered Microorganisms on a Paper Device

Kai Hong Guo; Pei Hsuan Chen; Chieh Lin; Chien Fu Chen; I. Ren Lee; Yi Chun Yeh

doi:10.1021/acssensors.7b00931

Determination of Gold Ions in Human Urine Using Genetically Engineered Microorganisms on a Paper Device

Kai Hong Guo, Pei Hsuan Chen, Chieh Lin, Chien Fu Chen, I. Ren Lee, Yi Chun Yeh^*

^*此作品的通信作者

化學系

研究成果: 雜誌貢獻 › 期刊論文 › 同行評審

19 引文斯高帕斯（Scopus）

摘要

This paper presents a whole-cell biosensor that operates in conjunction with a smartphone-based fluorescence diagnostic system on a paper device to monitor the concentration of gold ions in human urine. The heavy metal-tolerant bacteria Cupriavidus metallidurans was genetically engineered for use as a chassis in a red fluorescent protein (RFP)-based microbial sensor. The biosensor is highly sensitive to gold ions, with a detection limit of 110 nM. The proposed smartphone-based analysis system provides a user-friendly approach to design tools of personal health monitoring for reporting the presence of gold ions in human urine.

原文	英語
頁（從 - 到）	744-748
頁數	5
期刊	ACS Sensors
卷	3
發行號	4
DOIs	https://doi.org/10.1021/acssensors.7b00931
出版狀態	已發佈 - 2018 4月 27

ASJC Scopus subject areas

生物工程
儀器
製程化學與技術
流體流動和轉移過程

UN SDG

此研究成果有助於以下永續發展目標

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10.1021/acssensors.7b00931

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title = "Determination of Gold Ions in Human Urine Using Genetically Engineered Microorganisms on a Paper Device",

abstract = "This paper presents a whole-cell biosensor that operates in conjunction with a smartphone-based fluorescence diagnostic system on a paper device to monitor the concentration of gold ions in human urine. The heavy metal-tolerant bacteria Cupriavidus metallidurans was genetically engineered for use as a chassis in a red fluorescent protein (RFP)-based microbial sensor. The biosensor is highly sensitive to gold ions, with a detection limit of 110 nM. The proposed smartphone-based analysis system provides a user-friendly approach to design tools of personal health monitoring for reporting the presence of gold ions in human urine.",

keywords = "Cupriavidus metallidurans, cueR, paper device, smartphone-based diagnostic system, whole-cell biosensor",

author = "Guo, {Kai Hong} and Chen, {Pei Hsuan} and Chieh Lin and Chen, {Chien Fu} and Lee, {I. Ren} and Yeh, {Yi Chun}",

note = "Publisher Copyright: {\textcopyright} 2018 American Chemical Society.",

year = "2018",

month = apr,

day = "27",

doi = "10.1021/acssensors.7b00931",

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AU - Guo, Kai Hong

AU - Chen, Pei Hsuan

AU - Lin, Chieh

AU - Chen, Chien Fu

AU - Lee, I. Ren

AU - Yeh, Yi Chun

PY - 2018/4/27

Y1 - 2018/4/27

N2 - This paper presents a whole-cell biosensor that operates in conjunction with a smartphone-based fluorescence diagnostic system on a paper device to monitor the concentration of gold ions in human urine. The heavy metal-tolerant bacteria Cupriavidus metallidurans was genetically engineered for use as a chassis in a red fluorescent protein (RFP)-based microbial sensor. The biosensor is highly sensitive to gold ions, with a detection limit of 110 nM. The proposed smartphone-based analysis system provides a user-friendly approach to design tools of personal health monitoring for reporting the presence of gold ions in human urine.

AB - This paper presents a whole-cell biosensor that operates in conjunction with a smartphone-based fluorescence diagnostic system on a paper device to monitor the concentration of gold ions in human urine. The heavy metal-tolerant bacteria Cupriavidus metallidurans was genetically engineered for use as a chassis in a red fluorescent protein (RFP)-based microbial sensor. The biosensor is highly sensitive to gold ions, with a detection limit of 110 nM. The proposed smartphone-based analysis system provides a user-friendly approach to design tools of personal health monitoring for reporting the presence of gold ions in human urine.

KW - Cupriavidus metallidurans

KW - cueR

KW - paper device

KW - smartphone-based diagnostic system

KW - whole-cell biosensor

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Determination of Gold Ions in Human Urine Using Genetically Engineered Microorganisms on a Paper Device

摘要

ASJC Scopus subject areas

UN SDG

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