摘要
A simple, highly sensitive and specific method based on gas-chromatography-selected ion monitoring (SIM) mass spectrometry has been developed for the quantitation of corticosterone in rat and mouse plasma. After extraction of the plasma with ethyl acetate, the residue was trimethy-silylated with pentafluorobenzyl hydroxylamine-trimethylsilyl (PFBO-TMS). Detection of the derivatives was accomplished by a quadruple mass spectrometer in the selected ion monitoring mode (m/z of 316, 648, 663 and 678). The detection limit of the assay was 0.1 pg on column. The results show that in the plasma of non-stressed animals, only minor amounts of corticosterone were found; whereas in the plasma of stressed animals, it was dramatically increased. The method developed here can be used to examine corticosterone levels as a marker of stress in rats and mice and may also be used for estimation of the effect of stress-release medications.
原文 | 英語 |
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頁(從 - 到) | 93-101 |
頁數 | 9 |
期刊 | Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences |
卷 | 783 |
發行號 | 1 |
DOIs | |
出版狀態 | 已發佈 - 2003 1月 5 |
ASJC Scopus subject areas
- 分析化學
- 生物化學
- 臨床生物化學
- 細胞生物學