摘要
The separation and on-line concentration of corticosterone in mouse blood was achieved by means of capillary electrophoresis/UV absorbance using sodium dodecyl sulfate (SDS) as a surfactant. The procedure involved the use of an on-line sample concentration method by sweeping-micellar electrokinetic chromatography (sweeping-MEKC). Optimal on-line concentration and separation conditions were determined. The detection limit for this method was 5 ng/ml (S/N=3) and photodiode array detection at 247 nm was used for identification. For the analysis of actual samples, corticosterones from blood samples of a non-stressed and stressed mouse were determined. The results show that only a minor amount of corticosterone was produced by a non-stressed mouse, whereas a significant amount was present in the blood sample from a stressed mouse. The method developed here can be used to examine corticosterone levels as a marker of stress in test animals and may also be used for estimating the effect of stress-release medications.
原文 | 英語 |
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頁(從 - 到) | 317-325 |
頁數 | 9 |
期刊 | Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences |
卷 | 785 |
發行號 | 2 |
DOIs | |
出版狀態 | 已發佈 - 2003 3月 5 |
ASJC Scopus subject areas
- 分析化學
- 生物化學
- 臨床生物化學
- 細胞生物學