Determination of corticosterone in mouse plasma by a sweeping technique using micellar electrokinetic chromatography

Chun Hsueh Wu, Mei Chuan Chen, An Kai Su, Pin Yan Shu, Shiu Huey Chou, Cheng Huang Lin*

*此作品的通信作者

研究成果: 雜誌貢獻期刊論文同行評審

30 引文 斯高帕斯(Scopus)

摘要

The separation and on-line concentration of corticosterone in mouse blood was achieved by means of capillary electrophoresis/UV absorbance using sodium dodecyl sulfate (SDS) as a surfactant. The procedure involved the use of an on-line sample concentration method by sweeping-micellar electrokinetic chromatography (sweeping-MEKC). Optimal on-line concentration and separation conditions were determined. The detection limit for this method was 5 ng/ml (S/N=3) and photodiode array detection at 247 nm was used for identification. For the analysis of actual samples, corticosterones from blood samples of a non-stressed and stressed mouse were determined. The results show that only a minor amount of corticosterone was produced by a non-stressed mouse, whereas a significant amount was present in the blood sample from a stressed mouse. The method developed here can be used to examine corticosterone levels as a marker of stress in test animals and may also be used for estimating the effect of stress-release medications.

原文英語
頁(從 - 到)317-325
頁數9
期刊Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
785
發行號2
DOIs
出版狀態已發佈 - 2003 3月 5

ASJC Scopus subject areas

  • 分析化學
  • 生物化學
  • 臨床生物化學
  • 細胞生物學

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