Detection of pathogens using graphene quantum dots and gold nanoclusters on paper-based analytical devices

Hao Yuan, Jia Hui Lin, Zhi Shun Dong, Wei Ting Chen, Yau Kei Chan, Yi Chun Yeh, Huan Tsung Chang, Chien Fu Chen*

*此作品的通信作者

研究成果: 雜誌貢獻期刊論文同行評審

摘要

Pathogens are a persistent threat to human health, causing infectious disease and millions of deaths annually. The accurate and timely detection of pathogens is crucial in disease prevention, treatment, and monitoring. Although reliable detection methods are well established, many of them are still limited in use to clinical laboratories due to the need for costly and specialized instrumentation. In this study, we demonstrate a handheld and low-cost pathogen sensor consisting of a paper-based analytical device (μPAD) that can perform immunoassays and quantify analyte concentration by integrating with an automated color detection system that analyzes the color intensity of the μPAD. The core of the proposed sensor is the portable color detection system that can read the red-green-blue color of the paper emitted light from fluorescent nanomaterials, including graphene quantum dots (GQDs) and gold nanoclusters (AuNCs), which are conjugated with antibodies to indicate the immunoassay results, converting the presence of a pathogen to a colorful fluorescence signal. By adopting GQDs and AuNCs with high quantum yield and relatively high fluorescence intensity as the sensing signal, the paper-based detection system decreases the detection limit to as low as subnanogram/mL. Furthermore, GQDs and AuNCs can emit distinguishable fluorescence under the same light source (UV light) and possess limited background interference from the cellulose, enabling two or more analytes to be simultaneously detected with one UV light. Furthermore, a reaction time of just 10 min is needed, enabling diagnoses to be made in a timely manner and with high sensitivity. As a result, the proposed handheld pathogen sensor can rapidly detect the presence of pathogens with enhanced sensitivity and multiplexity, along with low instrumentation requirements, making it suitable for use in resource-limited settings where medical infrastructure is lacking.

原文英語
文章編號131824
期刊Sensors and Actuators, B: Chemical
363
DOIs
出版狀態已發佈 - 2022 7月 15

ASJC Scopus subject areas

  • 電子、光磁材料
  • 儀器
  • 凝聚態物理學
  • 表面、塗料和薄膜
  • 金屬和合金
  • 電氣與電子工程
  • 材料化學

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