摘要
The versatile Candida rugosa lipase (CRL) has been widely used in biotechnological applications. However, there have not been feasibility reports on the transesterification of non-edible oils to produce biodiesel using the commercial CRL preparations, mixtures of isozymes. In the present study, four liquid recombinant CRL isozymes (CRL1-CRL4) were investigated to convert various non-edible oils into biodiesel. The results showed that recombinant CRL2 and CRL4 exhibited superior catalytic efficiencies for producing fatty acid methyl ester (FAME) from Jatropha curcas seed oil. A maximum 95.3% FAME yield was achieved using CRL2 under the optimal conditions (50. wt% water, an initial 1. equivalent of methanol feeding, and an additional 0.5. equivalents of methanol feeding at 24. h for a total reaction time of 48. h at 37. °C). We concluded that specific recombinant CRL isozymes could be excellent biocatalysts for the biodiesel production from low-cost crude Jatropha oil.
原文 | 英語 |
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頁(從 - 到) | 54-59 |
頁數 | 6 |
期刊 | Bioresource Technology |
卷 | 192 |
DOIs | |
出版狀態 | 已發佈 - 2015 9月 1 |
ASJC Scopus subject areas
- 生物工程
- 環境工程
- 可再生能源、永續發展與環境
- 廢物管理和處置