Comparison of the use of anionic and cationic surfactants for the separation of steroids based on MEKC and sweeping-MEKC modes

Hui Ju Shen, Cheng Huang Lin*

*此作品的通信作者

研究成果: 雜誌貢獻期刊論文同行評審

27 引文 斯高帕斯(Scopus)

摘要

In attempts to improve the selectivity and sensitivity of steroid separation and to determine their migration order, comparison of the use of anionic and cationic surfactants based on the MEKC and sweeping-MEKC modes was made. A mixture of six steroids (progesterone, 17-hydroxy progesterone, 11-deoxycortisol, corticosterone, cortisone, and cortisol) could be separated and detected by means of the CE/UV-absorption method. The order of migration time for these steroids was compared under various conditions, including acidic/alkaline buffers, anionic/cationic surfactants, and positive/negative applied voltage, causing the direction of the EOF and the migration of micelles to change. The major rules for generally predicting the migration order of steroids are summarized. The detection limits were significantly improved when the sweeping-MEKC mode was applied.

原文英語
頁(從 - 到)1255-1262
頁數8
期刊Electrophoresis
27
發行號5-6
DOIs
出版狀態已發佈 - 2006 三月 1

ASJC Scopus subject areas

  • 分析化學
  • 生物化學
  • 臨床生物化學

指紋

深入研究「Comparison of the use of anionic and cationic surfactants for the separation of steroids based on MEKC and sweeping-MEKC modes」主題。共同形成了獨特的指紋。

引用此