Comparative effects of flavonoids on oxidant scavenging and ischemia-reperfusion injury in cardiomyocytes

Wei Tien Chang, Zuo Hui Shao, Jun Jie Yin, Sangeeta Mehendale, Chong Zhi Wang, Yimin Qin, Juan Li, Wen Jone Chen, Chiang Ting Chien, Lance B. Becker, Terry L. Vanden Hoek, Chun Su Yuan*

*此作品的通信作者

研究成果: 雜誌貢獻期刊論文同行評審

103 引文 斯高帕斯(Scopus)

摘要

Since flavonoids scavenge reactive oxygen species, they may potentially protect against ischemia/reperfusion injury. This study compared the scavenging capacity of specific flavonoids towards different reactive oxygen species. Whether the differential oxidant scavenging capacity correlated with their protective efficacy in ischemia/reperfusion injury of cardiomyocytes was determined. The free radical scavenging capacity of five flavonoids (wogonin, baicalin, baicalein, catechin and procyanidin B2) was analyzed using electron spin resonance spectrometry for 3 radicals: 1,1-diphenyl-2picrylhydrazyl (DPPH), superoxide and hydroxyl radical. A well-established chick cardiomyocyte model of ischemia (1 h)/reperfusion (3 h) was used to evaluate flavonoid-induced protection against ischemia/reperfusion injury in chronic treatment (pretreated 72 h and treated through ischemia/reperfusion) and acute treatment protocols (during ischemia/reperfusion or only at reperfusion). The cell viability was assessed by propidium iodide. The DPPH scavenging was most significant with catechin, followed by procyanidin B2, baicalein, baicalin, and wogonin. The superoxide scavenging was, similarly, most significant with catechin, followed by baicalein, procyanidin B2, and baicalin. For hydroxyl radical, only baicalein showed a significant scavenging capacity (> 50% reduction in ESR signal). For the cardiomyocyte studies, all flavonoids but wogonin showed protection against ischemia/reperfusion injury in the chronic treatment protocol. When flavonoids were administered only during ischemia/reperfusion, baicalein, procyanidin B2, and catechin significantly reduced cell death. If flavonoids were administered just at reperfusion, only baicalein and procyanidin B2 had protective effects, and the efficacy was less. Flavonoids possess specific but differential radical scavenging capacity, which, in conjunction with the timing of treatment, affects their protective efficacy in cardiomyocytes exposed to ischemia/reperfusion.

原文英語
頁(從 - 到)58-66
頁數9
期刊European Journal of Pharmacology
566
發行號1-3
DOIs
出版狀態已發佈 - 2007 7月 2
對外發佈

ASJC Scopus subject areas

  • 藥理

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