Colloid-gold nanoparticle enhanced detection sensitivity of paired surface plasma waves biosensor

Hsieh Ting Wu*, Yi Ling Chen, Yen Chen Huang, Wei Chih Liu, Ming Yaw Ng, Yin Fong Chang, Bao Yu Hsieh, Chien Chou

*此作品的通信作者

研究成果: 書貢獻/報告類型會議論文篇章

1 引文 斯高帕斯(Scopus)

摘要

Previous work with amplitude-sensitive paired surface plasma waves biosensor (PSPWB) demonstrated that the detection sensitivity of PSPWB is able to experimentally detect 0.001% sucrose-water solution and biomolecular interaction of 10pg/ml mouse IgG interacting with immobilized anti-mouse IgG successfully. Further development of the detection sensitivity of PSPWB has been conducted by using 20nm in diameter colloid gold nanoparticles conjugated with target molecules that can result in a higher mass coverage and a larger resonant angle change of plasmon resonance, thereby its detection sensitivity is further enhanced significantly. Bare gold nanoparticles, which is randomly suspended in solution, is adopted to differentiate biospecific binding induced further signal enhancement Experimentally, the sensitivity at 330fg/ml of Au-nanoparticle conjugated protein A (PA-Au) interacting with mouse IgG which is immobilized on a CM5 sensor chip was detected successfully. By this arrangement, 6-fold signal amplification is demonstrated compared with the same concentration of PA without conjugated gold nanoparticles.

原文英語
主出版物標題Nanoscale Imaging, Spectroscopy, Sensing, and Actuation for Biomedical Applications IV
DOIs
出版狀態已發佈 - 2007 四月 30
事件Nanoscale Imaging, Spectroscopy, Sensing, and Actuation for Biomedical Applications IV - San Jose, CA, 美国
持續時間: 2007 一月 232007 一月 24

出版系列

名字Progress in Biomedical Optics and Imaging - Proceedings of SPIE
6447
ISSN(列印)1605-7422

其他

其他Nanoscale Imaging, Spectroscopy, Sensing, and Actuation for Biomedical Applications IV
國家/地區美国
城市San Jose, CA
期間2007/01/232007/01/24

ASJC Scopus subject areas

  • 電子、光磁材料
  • 生物材料
  • 原子與分子物理與光學
  • 放射學、核子醫學和影像學

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