The determination of reaction mechanism of DNA cleavage using matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS) was discussed. The cleavage of HIV-27 was done by mixing 20 μM of HIV-27 and CoII complex in 20 μL H2O with 0.1% H2O2. The purification of HIV-27 and its cleavage products was done by ethanol precipitation, followed by denaturation. It was observed that the CoII complex was a bulge-specific probe to DNA structure, cleavaging at bulge site.
|出版狀態||已發佈 - 2002 十二月 1|
|事件||Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics - Orlando, FL, 美国|
持續時間: 2002 六月 2 → 2002 六月 6
|其他||Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics|
|期間||2002/06/02 → 2002/06/06|
ASJC Scopus subject areas