Characterization of bulge-specific DNA cleaver by matrix-assisted laser desorption inization time-of-flight mass spectrometry

Po Jui Chen, Sung Fang Chen, Yu Ju Chen

研究成果: 會議貢獻類型會議論文同行評審

摘要

The determination of reaction mechanism of DNA cleavage using matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS) was discussed. The cleavage of HIV-27 was done by mixing 20 μM of HIV-27 and CoII complex in 20 μL H2O with 0.1% H2O2. The purification of HIV-27 and its cleavage products was done by ethanol precipitation, followed by denaturation. It was observed that the CoII complex was a bulge-specific probe to DNA structure, cleavaging at bulge site.

原文英語
頁面435-436
頁數2
出版狀態已發佈 - 2002 十二月 1
事件Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics - Orlando, FL, 美国
持續時間: 2002 六月 22002 六月 6

其他

其他Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics
國家/地區美国
城市Orlando, FL
期間2002/06/022002/06/06

ASJC Scopus subject areas

  • 光譜

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